| TRANSSCLERAL ALBUMIN DIFFUSION AND SUPRACHOROIDAL ALBUMIN CONCENTRATION IN UVEAL EFFUSION SYNDROME. | |
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MedLine Citation:
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PMID: 21811210 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE:: To test the hypothesis that uveal effusion syndrome is caused by reduced transscleral albumin permeability. METHODS:: Surgical scleral specimens were obtained from a 55-year-old patient with nanophthalmic uveal effusion syndrome. Specimens were clamped in a modified Ussing chamber, and the rate of transscleral diffusion of fluorescein isothiocyanate-albumin was measured over 12 hours, using a spectrophotometer and predetermined standard curves. The diffusion coefficient was determined at 20°C, and then adjusted to body temperature using Einstein's equation. Results in 3 scleral samples were compared with 10 age-matched controls. Albumin and total protein concentration were measured in choroidal fluid and serum. RESULTS:: Histologic staining with Alcian blue showed interfibrillary acid mucin deposits. Transmission electron microscopy showed deposits measuring 1 μm to 10 μm and collections of expanded, degenerate collagen fibrils. The mean (±SD) albumin diffusion coefficient was 12% of that in controls (1.22 ± 0.67 × 10 vs. 10.3 ± 7.0 × 10 cm/second) and below the lower 95% confidence limit of the control group. The diffusion coefficient was calculated to increase 53% to 1.87 ± 1.03 × 10 cm/second at 37°C. Choroidal albumin concentration was much higher than physiologic levels, measuring 200 g/L (total protein 321 g/L), 5 times the serum albumin concentration of 42 g/L (total protein 70 g/L). CONCLUSION:: Nanophthalmic uveal effusion syndrome can be associated with reduced scleral permeability to albumin, and a very high concentration of retained suprachoroidal albumin. This will lead to an osmotic gradient that retains fluid and may partly explain the pathogenesis of uveal effusion syndrome in some patients. |
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Authors:
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Timothy L Jackson; Ali Hussain; Jon Salisbury; Roy Sherwood; Paul M Sullivan; John Marshall |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-29 |
Journal Detail:
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Title: Retina (Philadelphia, Pa.) Volume: - ISSN: 1539-2864 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-8-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309919 Medline TA: Retina Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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From the *Department of Ophthalmology, King's College Hospital, London, United Kingdom; †Biomedical Sciences, King's College London, London, United Kingdom; and ‡Vitreoretinal Unit, Moorfields Eye Hospital, London, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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