Document Detail


Transposase-derived proteins FHY3/FAR1 interact with PHYTOCHROME-INTERACTING FACTOR1 to regulate chlorophyll biosynthesis by modulating HEMB1 during deetiolation in Arabidopsis.
MedLine Citation:
PMID:  22634759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Successful chlorophyll biosynthesis during initial light exposure is critical for plant survival and growth, as excess accumulation of chlorophyll precursors in darkness can cause photooxidative damage to cells. Therefore, efficient mechanisms have evolved to precisely regulate chlorophyll biosynthesis in plants. Here, we identify FAR-RED ELONGATED HYPOCOTYL3 (FHY3) and FAR-RED IMPAIRED RESPONSE1 (FAR1), two transposase-derived transcription factors, as positive regulators of chlorophyll biosynthesis in Arabidopsis thaliana. We show that null mutations in FHY3 and FAR1 cause reduced protochlorophyllide (a precursor of chlorophyll) levels in darkness and less photobleaching in the light. We find that FHY3 directly binds to the promoter and activates expression of HEMB1, which encodes 5-aminolevulinic acid dehydratase in the chlorophyll biosynthetic pathway. We reveal that PHYTOCHROME-INTERACTING FACTOR1 physically interacts with the DNA binding domain of FHY3, thereby partly repressing FHY3/FAR1-activated HEMB1 expression. Strikingly, FHY3 expression is upregulated by white light. In addition, our genetic data indicate that overexpression, severe reduction, or lack of HEMB1 impairs plant growth and development. Together, our findings reveal a crucial role of FHY3/FAR1 in regulating chlorophyll biosynthesis, thus uncovering a new layer of regulation by which light promotes plant dark-light transition in early seedling development.
Authors:
Weijiang Tang; Wanqing Wang; Dongqin Chen; Qiang Ji; Yanjun Jing; Haiyang Wang; Rongcheng Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-25
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-06-28     Completed Date:  2012-10-25     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1984-2000     Citation Subset:  IM    
Affiliation:
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  metabolism*
Arabidopsis Proteins / genetics,  metabolism*
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism*
Chlorophyll / biosynthesis*
Nuclear Proteins / genetics,  metabolism*
Phytochrome / genetics,  metabolism*
Promoter Regions, Genetic / genetics
Protein Binding / genetics,  physiology
Seedling / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Basic Helix-Loop-Helix Transcription Factors; 0/FAR1 protein, Arabidopsis; 0/FHY3 protein, Arabidopsis; 0/Nuclear Proteins; 0/PIF1 protein, Arabidopsis; 11121-56-5/Phytochrome; 1406-65-1/Chlorophyll
Comments/Corrections

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