Document Detail


Transport of side-chain oxidized oxysterols in the human circulation.
MedLine Citation:
PMID:  9630709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the transport of 24-hydroxycholesterol, 27-hydroxycholesterol and 3beta-hydroxy-5-cholestenoic acid in the circulation, the distribution of these oxysterols was determined in plasma, very low density lipoprotein, low density lipoprotein, high density lipoprotein, and lipoprotein-free plasma. An accurate method based on isotope dilution-mass spectrometry with use of individual deuterium labeled internal standards was used. 24-Hydroxycholesterol and 27-hydroxycholesterol were found to be associated mainly with HDL and LDL, whereas 3beta-hydroxy-5-cholestenoic acid was found predominantly in the lipoprotein-free fraction. While both 24-hydroxycholesterol and 27-hydroxycholesterol are present mainly in esterified form in plasma, 3beta-hydroxy-5-cholestenoic acid was present as free acid only. For reasons of comparison, a number of other oxysterols were determined in plasma and in isolated lipoprotein fractions. Significant amounts of these oxysterols were formed by cholesterol autoxidation during fractionation of plasma. It was therefore not possible to calculate the distribution of these oxysterols in the different plasma fractions. The present results are consistent with our previous finding that the less polar cholesterol metabolite 27-hydroxycholesterol competes with cholesterol for transport out of cells using HDL as an acceptor molecule, whereas the transport of the more polar compound 3beta-hydroxy-5-cholestenoic acid is facilitated by albumin.
Authors:
A Babiker; U Diczfalusy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1392     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-30     Completed Date:  1998-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  333-9     Citation Subset:  IM    
Affiliation:
Department of Medical Laboratory Sciences and Technology, Division of Clinical Chemistry, C1.62, Karolinska Institute, Huddinge University Hospital, SE-141 86 Huddinge, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Transport
Cholesterol / analogs & derivatives*,  blood
Esterification
Female
Humans
Hydroxycholesterols / blood*
Indicator Dilution Techniques
Lipoproteins, HDL / blood
Lipoproteins, LDL / blood
Lipoproteins, VLDL / blood
Male
Mass Spectrometry
Middle Aged
Oxidation-Reduction
Chemical
Reg. No./Substance:
0/Hydroxycholesterols; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 20380-11-4/27-hydroxycholesterol; 474-73-7/24-hydroxycholesterol; 57-88-5/Cholesterol; 6561-58-6/3-hydroxy-5-cholestenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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