Document Detail

Transport of prostaglandin E1 across the blood-brain barrier in rats.
MedLine Citation:
PMID:  15638994     Owner:  NLM     Status:  MEDLINE    
The transport of prostaglandin E(1) (PGE(1)) across the blood-brain barrier (BBB) was characterized using an in-situ rat brain perfusion technique. The uptake of [(3)H]PGE(1) was not affected by shortchain monocarboxylic acids (butyric acid and valeric acid). On the other hand, uptake of [(3)H]PGE(1) was significantly inhibited by medium-chain monocarboxylic acids such as hexanoic acid, enanthic acid and octanoic acid. These medium-chain monocarboxylic acids showed a more potent inhibitory effect on [(3)H]PGE(1) uptake with increasing number of carbon atoms. In contrast, there was no decrease in [(3)H]PGE(1) transport by any dicarboxylic acids with 5-8 carbon atoms. Valproic acid decreased [(3)H]PGE(1) uptake, whereas p-aminohippuric acid, a substrate for the organic anion transporter family, did not inhibit [(3)H]PGE(1) transport. Bromocresol green, an inhibitor of prostaglandin transporter (PGT), strongly decreased [(3)H]PGE(1) transport across the BBB. In addition, digoxin and taurocholate, substrates for organic anion transporting polypeptide subtype 2 (Oatp2), significantly inhibited [(3)H]PGE(1) uptake. RT-PCR analysis revealed that PGT mRNA and Oatp2 mRNA are expressed in a capillary-rich fraction from rat brain. Thus, it is suggested that PGE(1) transport across the BBB is mediated by some specific transport systems, possibly by the members of the Oatp family.
T Taogoshi; A Nomura; T Murakami; J Nagai; M Takano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  57     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-10     Completed Date:  2005-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM    
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
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MeSH Terms
Alkaline Phosphatase / metabolism
Alprostadil / pharmacokinetics*
Antiporters / biosynthesis
Biological Transport, Active
Blood-Brain Barrier*
Bromcresol Green / pharmacology
Carboxylic Acids / pharmacology
Cardiotonic Agents / pharmacology
Cholagogues and Choleretics / pharmacology
DNA-Binding Proteins / biosynthesis
Digoxin / pharmacology
Organic Anion Transporters
Organic Cation Transport Proteins / biosynthesis
RNA, Messenger / biosynthesis,  genetics
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Taurocholic Acid / pharmacology
Reg. No./Substance:
0/Antiporters; 0/Carboxylic Acids; 0/Cardiotonic Agents; 0/Cholagogues and Choleretics; 0/DNA-Binding Proteins; 0/Organic Anion Transporters; 0/Organic Cation Transport Proteins; 0/RNA, Messenger; 0/Slco1a4 protein, rat; 0/Slco1c1 protein, rat; 0/Slco2a1 protein, rat; 20830-75-5/Digoxin; 745-65-3/Alprostadil; 76-60-8/Bromcresol Green; 81-24-3/Taurocholic Acid; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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