Document Detail


Transport of prostaglandin E1 across the blood-brain barrier in rats.
MedLine Citation:
PMID:  15638994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transport of prostaglandin E(1) (PGE(1)) across the blood-brain barrier (BBB) was characterized using an in-situ rat brain perfusion technique. The uptake of [(3)H]PGE(1) was not affected by shortchain monocarboxylic acids (butyric acid and valeric acid). On the other hand, uptake of [(3)H]PGE(1) was significantly inhibited by medium-chain monocarboxylic acids such as hexanoic acid, enanthic acid and octanoic acid. These medium-chain monocarboxylic acids showed a more potent inhibitory effect on [(3)H]PGE(1) uptake with increasing number of carbon atoms. In contrast, there was no decrease in [(3)H]PGE(1) transport by any dicarboxylic acids with 5-8 carbon atoms. Valproic acid decreased [(3)H]PGE(1) uptake, whereas p-aminohippuric acid, a substrate for the organic anion transporter family, did not inhibit [(3)H]PGE(1) transport. Bromocresol green, an inhibitor of prostaglandin transporter (PGT), strongly decreased [(3)H]PGE(1) transport across the BBB. In addition, digoxin and taurocholate, substrates for organic anion transporting polypeptide subtype 2 (Oatp2), significantly inhibited [(3)H]PGE(1) uptake. RT-PCR analysis revealed that PGT mRNA and Oatp2 mRNA are expressed in a capillary-rich fraction from rat brain. Thus, it is suggested that PGE(1) transport across the BBB is mediated by some specific transport systems, possibly by the members of the Oatp family.
Authors:
T Taogoshi; A Nomura; T Murakami; J Nagai; M Takano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  57     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-10     Completed Date:  2005-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Alprostadil / pharmacokinetics*
Animals
Antiporters / biosynthesis
Biological Transport, Active
Blood-Brain Barrier*
Bromcresol Green / pharmacology
Capillaries
Carboxylic Acids / pharmacology
Cardiotonic Agents / pharmacology
Cholagogues and Choleretics / pharmacology
DNA-Binding Proteins / biosynthesis
Digoxin / pharmacology
Male
Organic Anion Transporters
Organic Cation Transport Proteins / biosynthesis
Perfusion
Permeability
RNA, Messenger / biosynthesis,  genetics
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Taurocholic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Antiporters; 0/Carboxylic Acids; 0/Cardiotonic Agents; 0/Cholagogues and Choleretics; 0/DNA-Binding Proteins; 0/Organic Anion Transporters; 0/Organic Cation Transport Proteins; 0/RNA, Messenger; 0/Slco1a4 protein, rat; 0/Slco1c1 protein, rat; 0/Slco2a1 protein, rat; 20830-75-5/Digoxin; 745-65-3/Alprostadil; 76-60-8/Bromcresol Green; 81-24-3/Taurocholic Acid; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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