Document Detail


Transport properties of toad kidney epithelia in culture.
MedLine Citation:
PMID:  6269434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characteristics of a continuous line of toad kidney epithelial cells (A6) are described. These cells form a monolayer epithelium of high transepithelial electrical resistance (about 5,000 omega . cm2). The cells generate a transepithelial potential difference (apical surface negative) of about 9 mV. The short-circuit current is equivalent to net sodium flux. Net sodium flux is stimulated by aldosterone and by analogues of cAMP. The stimulation is readily reversible. Neither urea permeability nor osmotic water flow is altered by analogues of cAMP. Amiloride eliminates 90% of the short-circuit current. Thus A6 cells form an epithelium with several differentiated properties including hormonal regulation of transport.
Authors:
F M Perkins; J S Handler
Related Documents :
10747184 - The role of clc-3 in volume-activated chloride currents and volume regulation in bovine...
1241334 - Ionic determinants of spontaneous activity in clusters of cultured cardiac cells from n...
22504664 - Hedgehog signalling stimulates precursor cell accumulation and impairs epithelial matur...
21302734 - Standard oral rehydration solution as a new storage medium for avulsed teeth.
24371614 - An unusual case of fallopian tube intraepithelial carcinoma with mucinous goblet cell d...
3892474 - Responses of enzymatically isolated mammalian vascular smooth muscle cells to pharmacol...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  241     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C154-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
8-Bromo Cyclic Adenosine Monophosphate
Aldosterone / pharmacology
Animals
Biological Transport
Cell Line
Cyclic AMP / analogs & derivatives,  pharmacology
Epithelium / drug effects,  physiology,  ultrastructure
Kidney / drug effects,  physiology*
Kinetics
Membrane Potentials / drug effects
Permeability
Sodium / metabolism
Sucrose / metabolism
Thionucleotides / pharmacology
Urea / metabolism
Xenopus
Grant Support
ID/Acronym/Agency:
1F32AM05879-01/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Thionucleotides; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 41941-66-6/8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; 52-39-1/Aldosterone; 57-13-6/Urea; 57-50-1/Sucrose; 60-92-4/Cyclic AMP; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Separate turnover of cytochrome c and myoglobin in the red types of skeletal muscle.
Next Document:  FSH and testosterone effects in seminiferous tubules of immature hypophysectomized rats.