Document Detail

Transport properties of toad kidney epithelia in culture.
MedLine Citation:
PMID:  6269434     Owner:  NLM     Status:  MEDLINE    
The characteristics of a continuous line of toad kidney epithelial cells (A6) are described. These cells form a monolayer epithelium of high transepithelial electrical resistance (about 5,000 omega . cm2). The cells generate a transepithelial potential difference (apical surface negative) of about 9 mV. The short-circuit current is equivalent to net sodium flux. Net sodium flux is stimulated by aldosterone and by analogues of cAMP. The stimulation is readily reversible. Neither urea permeability nor osmotic water flow is altered by analogues of cAMP. Amiloride eliminates 90% of the short-circuit current. Thus A6 cells form an epithelium with several differentiated properties including hormonal regulation of transport.
F M Perkins; J S Handler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  241     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C154-9     Citation Subset:  IM    
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate
Aldosterone / pharmacology
Biological Transport
Cell Line
Cyclic AMP / analogs & derivatives,  pharmacology
Epithelium / drug effects,  physiology,  ultrastructure
Kidney / drug effects,  physiology*
Membrane Potentials / drug effects
Sodium / metabolism
Sucrose / metabolism
Thionucleotides / pharmacology
Urea / metabolism
Grant Support
Reg. No./Substance:
0/Thionucleotides; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 41941-66-6/8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; 52-39-1/Aldosterone; 57-13-6/Urea; 57-50-1/Sucrose; 60-92-4/Cyclic AMP; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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