Document Detail


Transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy.
MedLine Citation:
PMID:  23035662     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aim: The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum. Materials & methods: With single-particle tracking microscopy, the mobility of 0.1 and 0.2 µm fluorescent polyethylene glycol (PEG) modified, carboxylate- and N,N-dimethylethylenediamine-modified polystyrene nanospheres were evaluated in fresh cystic fibrosis sputum, as well as Burkholderia multivorans and Pseudomonas aeruginosa biofilms. Results: PEGylation increased the mobility of the particles in sputum and biofilms, while the charged nanospheres were strongly immobilized. However, the transport of the PEGylated nanoparticles was lower in sputum compared with biofilms. Furthermore, the particle transport showed heterogeneity in samples originating from different patients. Conclusion: This study's data suggest that for future nanocarrier design it will be essential to combine PEGylation with a targeting moiety to ensure sufficient mobility in mucus and a better accumulation of the nanoparticles in the biofilm. Original submitted 14 February 2012; Revised submitted 24 July 2012.
Authors:
Katrien Forier; Anne-Sophie Messiaen; Koen Raemdonck; Hendrik Deschout; Joanna Rejman; Frans De Baets; Hans Nelis; Stefaan C De Smedt; Joseph Demeester; Tom Coenye; Kevin Braeckmans
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  -     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Ghent University, Harelbekestraat 72, 9000 Ghent, Ghent, Belgium.
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