| Transport of molybdate by Clostridium pasteurianum. | |
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MedLine Citation:
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PMID: 364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transport of 99MoO42- into dinitrogen-fixing cells of Clostridium pasteurianum was investigated. Transport of molybdate in this organism is energy dependent; sucrose is required in the minimal media, and the system is inhibited by the glycolysis inhibitors, NaF, iodoacetic acid, and arsenate. The cells accumulate molybdate against a concentration gradient, and the uptake shows a marked dependence on temperature (optimum 37 C) and pH (optimum 6.0). The rate of molybdate uptake with increasing molybdate concentrations shows saturation kinetics with an apparent Km and Vmax of 4.8 X 10(-5) M and 55 nmol/g of dry cells per min, respectively. Inhibition studies with the anions SO42-, S2O32-, WO42-, and VO32- show that SO42- and WO42- competitively inhibit MoO42- uptake (apparent Ki [SO42-] is 3.0 X 10(-5) M; apparent Ki [WO42-] is 2.4 X 10(-5), whereas S2O32- and VO32- have no inhibitory effect. Exchange experiments with MoO42- show that only a small percentage of the 99MoO42- taken up by the cells is exchangeable. Exchange experiments with WO42- and SO42- indicate that once inside the cells WO42- and SO42- cannot substitute for MoO42-. |
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Authors:
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B B Elliott; L E Mortenson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 124 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1975 Dec |
Date Detail:
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Created Date: 1976-02-20 Completed Date: 1976-02-20 Revised Date: 2010-08-31 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1295-1301 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Arsenates / pharmacology Biological Transport, Active Clostridium / metabolism* Fluorides / pharmacology Hydrogen-Ion Concentration Iodoacetates / pharmacology Kinetics Molybdenum / metabolism* Pyruvates / metabolism Sucrose / metabolism Sulfates / pharmacology Temperature Thiosulfates / pharmacology Tungsten / pharmacology Vanadium / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Arsenates; 0/Fluorides; 0/Iodoacetates; 0/Pyruvates; 0/Sulfates; 0/Thiosulfates; 57-50-1/Sucrose; 7439-98-7/Molybdenum; 7440-33-7/Tungsten; 7440-62-2/Vanadium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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