Document Detail


Transport and metabolism of pantothenic acid by rat kidney.
MedLine Citation:
PMID:  6696896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 microM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.
Authors:
L M Karnitz; C J Gross; L M Henderson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  769     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-04-04     Completed Date:  1984-04-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  486-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Kidney / metabolism*,  ultrastructure
Male
Microvilli / metabolism
Pantothenic Acid / metabolism*
Penicillins / pharmacology
Perfusion
Rats
Rats, Inbred Strains
Sodium / metabolism
Grant Support
ID/Acronym/Agency:
5 R01-AM19012/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Penicillins; 7440-23-5/Sodium; 79-83-4/Pantothenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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