Document Detail


Transport and metabolism of exogenous fumarate and 3-phosphoglycerate in vascular smooth muscle.
MedLine Citation:
PMID:  10395075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The keto (linear) form of exogenous fructose 1,6-bisphosphate, a highly charged glycolytic intermediate, may utilize a dicarboxylate transporter to cross the cell membrane, support glycolysis, and produce ATP anaerobically. We tested the hypothesis that fumarate, a dicarboxylate, and 3-phosphoglycerate (3-PG), an intermediate structurally similar to a dicarboxylate, can support contraction in vascular smooth muscle during hypoxia. To assess ATP production during hypoxia we measured isometric force maintenance in hog carotid arteries during hypoxia in the presence or absence of 20 mM fumarate or 3-PG. 3-PG improved maintenance of force (p < 0.05) during the 30-80 min period of hypoxia. Fumarate decreased peak isometric force development by 9.5% (p = 0.008) but modestly improved maintenance of force (p < 0.05) throughout the first 80 min of hypoxia. 13C-NMR on tissue extracts and superfusates revealed 1,2,3,4-(13)C-fumarate (5 mM) metabolism to 1,2,3,4-(13)C-malate under oxygenated and hypoxic conditions suggesting uptake and metabolism of fumarate. In conclusion, exogenous fumarate and 3-PG readily enter vascular smooth muscle cells, presumably by a dicarboxylate transporter, and support energetically important pathways.
Authors:
D R Finder; C D Hardin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  195     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-10-28     Completed Date:  1999-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-21     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Missouri, Columbia 65212, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia
Biological Transport / physiology
Carbon Isotopes
Carotid Arteries
Cell Membrane / metabolism
Fumarates / metabolism*
Glyceric Acids / metabolism*
Muscle, Smooth, Vascular / metabolism*
Nuclear Magnetic Resonance, Biomolecular
Swine
Time Factors
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fumarates; 0/Glyceric Acids; 110-17-8/fumaric acid; 820-11-1/3-phosphoglycerate

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