| Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines. | |
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MedLine Citation:
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PMID: 8207055 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have compared the intracellular transport and subcellular distribution of MHC class II-invariant chain complexes in a wild-type HLA-DR3 homozygous cell line and a mutant cell line, T2.DR3. The latter has a defect in antigen processing and accumulates HLA-DR3 molecules associated with an invariant chain-derived peptide (CLIP) rather than the normal complement of peptides derived from endocytosed proteins. We find that in the wild-type cells, CLIP is transiently associated with HLA-DR3 molecules, suggesting that the peptide is a normal class II-associated intermediate generated during proteolysis of the invariant chain. In the mutant cell line proteolysis of the invariant chain is less efficient, and HLA-DR3/CLIP complexes are generated much more slowly. Examination of the mutant cell line by immunoelectronmicroscopy shows that class II-invariant chain complexes accumulate intracellularly in large acidic vesicles which contain lysosomal markers, including beta-hexosaminidase, cathepsin D, and the lysosomal membrane protein CD63. The markers in these vesicles are identical to those seen in the class II-containing vesicles (MIICs) seen in the wild-type cells but the morphology is drastically different. The vesicles in the mutant cells are endocytic, as measured by the internalization of BSA-gold conjugates. The implication of these findings for antigen processing in general and the nature of the mutation in particular are discussed. |
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Authors:
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J M Riberdy; R R Avva; H J Geuze; P Cresswell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 125 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1994 Jun |
Date Detail:
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Created Date: 1994-07-12 Completed Date: 1994-07-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1225-37 Citation Subset: IM |
Affiliation:
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Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigen Presentation
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genetics* Antigens, Differentiation, B-Lymphocyte* B-Lymphocytes / immunology*, ultrastructure Biological Markers Biological Transport Cell Line Endocytosis Frozen Sections HLA-DR3 Antigen / genetics, isolation & purification, metabolism Histocompatibility Antigens Class II / genetics, isolation & purification, metabolism* Homozygote Humans Immunohistochemistry Microscopy, Immunoelectron Mutation Peptide Fragments / isolation & purification, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI23081/AI/NIAID NIH HHS; NWO PG 900-523-094/PG/OAPP OPHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation, B-Lymphocyte; 0/Biological Markers; 0/HLA-DR3 Antigen; 0/Histocompatibility Antigens Class II; 0/Peptide Fragments; 0/invariant chain |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol Journal ID (publisher-id): J. Cell Biol. ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 6 Year: 1994 Volume: 125 Issue: 6 First Page: 1225 Last Page: 1237 ID: 2290929 Publisher Id: 94266956 PubMed Id: 8207055 |
| Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines | |
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