Document Detail


Transport in lymphatic capillaries. II. Microscopic velocity measurement with fluorescence photobleaching.
MedLine Citation:
PMID:  8769769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite its relevance to the physiology of lymph formation and propulsion, the instantaneous flow velocity in single lymphatic capillaries has not been measured to date. The method of fluorescence recovery after photobleaching (FRAP) was adapted for this purpose and used to characterize flow in the lymphatic capillaries in tail skin of anesthetized mice during a constant-pressure intradermal injection of fluorescein isothiocyanate-dextran (mol wt 2 x 10(6). The median lymph flow velocity was 4.7 microns/s, and the velocity magnitude ranged from 0 to 29 microns/s. The direction of flow was generally proximal, but stasis and backflow toward the site of injection was also detected. Evidence for oscillatory flow was detected in some FRAP experiments, and in separate experiments a periodicity of approximately 120 min-1, directly correlated to respiration frequency, was measured by tracking the motion of fluorescent latex microspheres (1 micron diam) introduced into the lymphatic capillary network. The velocity magnitude showed a correlation with duration of infusion but not with distance from injection site. It is speculated that the temporal decay of mean velocity magnitude could be related to the relaxation of local pressure gradients as partially collapsed vessels expand during the infusion.
Authors:
D A Berk; M A Swartz; A J Leu; R K Jain
Related Documents :
21740609 - Quantification of left-to-right shunt through patent ductus arteriosus by colour dopple...
24578539 - A cross-sectional study to compare two blood collection methods: direct venous puncture...
22477619 - Arteritic anterior ischemic optic neuropathy treated with intravenous prostaglandin e(1...
7261039 - Morphology and vascular anatomy of the gills of a primitive air-breathing fish, the bow...
2237309 - Superficial venous anatomy of the human palm.
6597629 - Aspiration in oral local anaesthesia. frequency of blood in cartridges in an undergradu...
16113889 - Suppression of brugia malayi (sub-periodic) larval development in aedes aegypti (liverp...
11715949 - Technology evaluation: gene therapy (fgf-5), vical.
12558999 - Correlation of local changes in extracellular oxygen and ph that accompany dopaminergic...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  270     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-12-20     Completed Date:  1996-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H330-7     Citation Subset:  IM    
Affiliation:
Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachuseits General Hospital, Boston, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Female
Fourier Analysis
Image Processing, Computer-Assisted
Lasers
Lymphatic System / physiology*
Mice
Mice, Nude
Microscopy, Fluorescence
Microspheres
Skin
Tail
Time Factors
Grant Support
ID/Acronym/Agency:
CA-56591/CA/NCI NIH HHS; CA-59255/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transport in lymphatic capillaries. I. Macroscopic measurements using residence time distribution th...
Next Document:  End-systolic pressure-volume relationship and intracellular control of contraction.