Document Detail


Transport and compartmentation of phosphite in higher plant cells--kinetic and P nuclear magnetic resonance studies.
MedLine Citation:
PMID:  18657056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphite (Phi, H(2)PO(3)(-)), being the active part of several fungicides, has been shown to influence not only the fungal metabolism but also the development of phosphate-deficient plants. However, the mechanism of phosphite effects on plants is still widely unknown. In this paper we analysed uptake, subcellular distribution and metabolic effects of Phi in tobacco BY-2 cells using in vivo(31)P nuclear magnetic resonance ((31)P-NMR) spectroscopy. Based on the kinetic properties of the phosphate transport system of tobacco BY-2 cells, it was demonstrated that phosphite inhibited phosphate uptake in a competitive manner. To directly follow the fate of phosphate and phosphite in cytoplasmic and vacuolar pools of tobacco cells, we took advantage of the pH-sensitive chemical shift of the Phi anion. The NMR studies showed a distinct cytoplasmic accumulation of Phi in Pi-deprived cells, whereas Pi resupply resulted in a rapid efflux of Phi. Pi-preloaded cells shifted Phi directly into vacuoles. These studies allowed for the first time to follow Phi flux processes in an in vivo setting in plants. On the other hand, the external Pi nutrition status and the metabolic state of the cells had a strong influence on the intracellular compartmentalization of xenobiotic Phi.
Authors:
Ralitza Danova-Alt; Cor Dijkema; Pieter DE Waard; Margret Köck
Related Documents :
8916216 - A novel technique for studying cellular function in human placenta: gestational changes...
20572856 - Intracellular ca2+ responses and cell volume regulation upon cholinergic and purinergic...
2473316 - Stimulation of the na+, k+ pump and the (na+, k+, cl-) cotransport system by endothelin...
1662906 - Intracellular ph in the ok cell. i. identification of h+ conductance and observations o...
3929546 - An attempt to influence dna content in postmitotic purkinje cells of the cerebellum.
9500236 - Comparative immunohistochemical study of stellate cells in normal canine and equine ade...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-24
Journal Detail:
Title:  Plant, cell & environment     Volume:  31     ISSN:  1365-3040     ISO Abbreviation:  Plant Cell Environ.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-10     Completed Date:  2008-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309004     Medline TA:  Plant Cell Environ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1510-21     Citation Subset:  IM    
Affiliation:
Martin Luther University of Halle-Wittenberg, Biocenter, 06120 Halle, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cell Compartmentation*
Cells, Cultured
Cytoplasm / metabolism
Kinetics
Magnetic Resonance Spectroscopy / methods
Phosphates / metabolism
Phosphites / metabolism*
Tobacco / metabolism*
Vacuoles / metabolism
Chemical
Reg. No./Substance:
0/Phosphates; 0/Phosphites

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular mechanisms underlying plant memory in JA-mediated defence responses.
Next Document:  The hydraulic architecture of Juniperus communis L. ssp. communis: shrubs and trees compared.