Transport of autophagosomes in neurites of PC12 cells during serum deprivation. | |
MedLine Citation:
|
PMID: 18094609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Autophagy has been linked to various human diseases, including many neurodegenerative disorders. The induction of autophagy has been detected in degenerating neurites initiated by different experimental paradigms and hence is of major interest. Axonal and dendritic degeneration was significantly delayed either by the application of the autophagy inhibitor 3-methyladenine (3-MA) or by knocking down the key autophagy-related genes Atg7 and Beclin 1. In addition, Tomato-LC3-labelled autophagosomes accumulate in neuritic beadings of PC12 cells during nerve growth factor (NGF) deprivation, which might be due to the failure of neurite transport. However, little is known about routes and dynamics of autophagosomes in the neurites of living cells. Here, we further demonstrate that LC3-labelled small autophagosomes are motile and move along the neurites of PC12 cells in both anterograde and retrograde directions after serum deprivation. The autophagosomes paused, re-started, and sometimes changed directions. These results provide valuable insight into neuritic transport of autophagosomes and imply a close relationship between the autophagic process and neurite degeneration. |
Authors:
|
Yi Yang; Kedi Xu; Tatsuro Koike; Xiaoxiang Zheng |
Related Documents
:
|
7766849 - Ngf-dependent neurotrophic-like effects of af102b, an m1 muscarinic agonist, in pc12m1 ... 9262149 - Differentiated b104 neuroblastoma cells are a high-resolution assay for micropatterned ... 2826369 - Nuclear receptors for 1,25(oh)2 vitamin d3 in thymus reticular cells studied by autorad... 14737019 - Possible role of interleukin-6 in pc12 cell death induced by mpp+ and tetrahydroisoquin... 8858629 - Nicotine rescues pc12 cells from death induced by nerve growth factor deprivation. 20654569 - An in vitro model for the assessment of manganese neurotoxicity. 8797939 - The nature and significance of liver cell vacuolation following hepatocellular injury--... 12584329 - Nef-mediated disruption of hla-a2 transport to the cell surface in t cells. 23359139 - Imbalance of desmoplastic stromal cell numbers drives aggressive cancer processes. |
Publication Detail:
|
Type: Journal Article; Review Date: 2007-12-14 |
Journal Detail:
|
Title: Autophagy Volume: 4 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2008 Feb |
Date Detail:
|
Created Date: 2008-01-21 Completed Date: 2008-03-19 Revised Date: 2011-06-30 |
Medline Journal Info:
|
Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
|
Languages: eng Pagination: 243-5 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, P.R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autophagy / physiology Biological Transport Culture Media, Serum-Free / pharmacology* Models, Biological Nerve Degeneration / etiology, metabolism, pathology Neurites / drug effects*, metabolism* PC12 Cells Phagosomes / metabolism* Rats |
Chemical | |
Reg. No./Substance:
|
0/Culture Media, Serum-Free |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yea...
Next Document: Potential subversion of autophagosomal pathway by picornaviruses.