Document Detail


Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acidic environments.
MedLine Citation:
PMID:  8795203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion.
Authors:
M J Sousa; L Miranda; M Côrte-Real; C Leão
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-17     Completed Date:  1996-10-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3152-7     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Minho, Braga, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid
Acetic Acids / pharmacokinetics*
Biological Transport / drug effects
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
Ethanol / pharmacology
Hydrogen-Ion Concentration
Saccharomycetales / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Acetic Acids; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 64-17-5/Ethanol; 64-19-7/Acetic Acid
Comments/Corrections

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