Document Detail


Transport of L: -proline by the proton-coupled amino acid transporter PAT2 in differentiated 3T3-L1 cells.
MedLine Citation:
PMID:  22711289     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mechanism and substrate specificity of the proton-coupled amino acid transporter 2 (PAT2, SLC36A2) have been studied so far only in heterologous expression systems such as HeLa cells and Xenopus laevis oocytes. In this study, we describe the identification of the first cell line that expresses PAT2. We cultured 3T3-L1 cells for up to 2 weeks and differentiated the cells into adipocytes in supplemented media containing 2 μM rosiglitazone. During the 14 day differentiation period the uptake of the prototype PAT2 substrate L: -[(3)H]proline increased ~5-fold. The macro- and microscopically apparent differentiation of 3T3-L1 cells coincided with their H(+) gradient-stimulated uptake of L: -[(3)H]proline. Uptake was rapid, independent of a Na(+) gradient but stimulated by an inwardly directed H(+) gradient with maximal uptake occurring at pH 6.0. L: -Proline uptake was found to be mediated by a transport system with a Michaelis constant (K(t)) of 130 ± 10 μM and a maximal transport velocity of 4.9 ± 0.2 nmol × 5 min(-1 )mg of protein(-1). Glycine, L: -alanine, and L: -tryptophan strongly inhibited L: -proline uptake indicating that these amino acids also interact with the transport system. It is concluded that 3T3-L1 adipocytes express the H(+)-amino acid cotransport system PAT2.
Authors:
Katja Zebisch; Matthias Brandsch
Related Documents :
523959 - Metabolism in hypothermically perfused human kidney carcinoma: utilization and producti...
1335399 - Post-operative changes in hepatic, intestinal, splenic and muscle fluxes of amino acids...
10195919 - Uptake of 13-hydroperoxylinoleic acid by cultured cells.
21637909 - A guide to temporal fossa augmentation with small gel particle hyaluronic acid dermal f...
19604939 - Amino acid inhibition of sodium-dependent synaptosomal proline uptake.
8833189 - Endothelin-1 regulates glucose utilization in cultured astrocytes by controlling interc...
15108979 - Effect of amino acid infusion on central thermoregulatory control in humans.
1265799 - New bile alcohols - synthesis of (22r)- and (22s)-5beta-cholestane-3alpha,7alpha,12alph...
15311949 - The enantioselective synthesis of alpha-amino acids by phase-transfer catalysis with ac...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-19
Journal Detail:
Title:  Amino acids     Volume:  -     ISSN:  1438-2199     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Biozentrum of the Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Natural orbital Fukui function and application in understanding cycloaddition reaction mechanisms.
Next Document:  Impaired self-awareness of motor deficits in Parkinson's disease: Association with motor asymmetry a...