Document Detail

Transport of L-citrulline in neural cell cultures.
MedLine Citation:
PMID:  11111155     Owner:  NLM     Status:  MEDLINE    
Uptake of L-[(14)C]citrulline was studied in cell culture models of the main neural cell populations, in astroglia-rich primary cultures derived from neonatal rat brain, in rat glioma cells C6-BU-1, in cells of the murine microglial clone N11 and in the glioma x neuroblastoma hybrid cell line 108CC15 with neuronal properties. For comparison, cells of the peripheral macrophage cell line RAW 264.7 were also investigated. A saturable component of uptake was found in all cases with K(M) values between 0.4 and 3.4 mM and V(max) values between 15 and 35 nmol.min(-1).(mg protein)(-1). A nonsaturable component dominated uptake at high concentrations of extracellular citrulline. Rates of uptake of L-citrulline were not affected when Na(+) or Cl(-) were omitted from the incubation medium or in the presence of depolarizing concentrations of K(+). Saturable uptake of citrulline was strongly inhibited by an excess of histidine or beta-2-aminobicyclo-(2.2.1)-heptane-2-carboxylic acid; excess amounts of arginine, creatine, glutamate, cysteic acid or N-methyl-alpha-aminoisobutyric acid did not reduce citrulline uptake. Preincubation of the cells with bacterial lipopolysaccharide and interferon gamma did not stimulate transport of citrulline. The results suggest that at physiological concentrations citrulline is taken up by neural cells with the help of transport system L for large neutral amino acids. Therefore, in the brain, effective utilization of extracellular citrulline as part of an intercellular trafficking of intermediates of an NO/citrulline cycle depends on the concentrations of all neutral amino acids present.
A Schmidlin; S Fischer; H Wiesinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental neuroscience     Volume:  22     ISSN:  0378-5866     ISO Abbreviation:  Dev. Neurosci.     Publication Date:    2000 Sep-Dec
Date Detail:
Created Date:  2001-02-01     Completed Date:  2001-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7809375     Medline TA:  Dev Neurosci     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  393-8     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel
Physiologisch-Chemisches Institut, Universität Tübingen, Deutschland.
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MeSH Terms
Biological Transport / drug effects,  physiology
Cells, Cultured
Chlorides / metabolism
Citrulline / metabolism*,  pharmacokinetics
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Glioma / metabolism
Interferon-gamma / pharmacology
Lipopolysaccharides / pharmacology
Macrophages / cytology,  drug effects,  metabolism
Neuroglia / cytology,  metabolism
Neurons / cytology,  metabolism*
Nitric Oxide Synthase / antagonists & inhibitors
Potassium / metabolism
Rats, Wistar
Sodium / metabolism
Reg. No./Substance:
0/Chlorides; 0/Enzyme Inhibitors; 0/Lipopolysaccharides; 372-75-8/Citrulline; 7440-09-7/Potassium; 7440-23-5/Sodium; 82115-62-6/Interferon-gamma; EC Oxide Synthase

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