Document Detail

Transport of 4-hydroxyphenylacetic acid in Klebsiella pneumoniae.
MedLine Citation:
PMID:  1731619     Owner:  NLM     Status:  MEDLINE    
Klebsiella pneumoniae M5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-HPA). This transport system has a Kt of 16.3 microM and a maximal velocity of 31.2 nmol/min (milligrams dry weight). The transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular ATP concentrations, and the 4-HPA binding activity has been detected in the crude shock extracts. All these observations indicate that 4-HPA uptake is an active transport which involves a periplasmic binding protein and it seems to be energized by phosphate bond energy.
J L Allende; A Gibello; M Martin; A Garrido-Pertierra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  292     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-18     Completed Date:  1992-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-8     Citation Subset:  IM    
Departamento de Bioquímica y Biologiá Molecular IV, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
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MeSH Terms
Adenosine Triphosphate / metabolism
Biological Transport
Carbon Radioisotopes
Energy Metabolism
Klebsiella pneumoniae / growth & development,  metabolism*
Phenylacetates / metabolism*
Radioisotope Dilution Technique
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Phenylacetates; 156-38-7/4-hydroxyphenylacetic acid; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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