| Transport of [125I]transferrin through the rat blood-brain barrier. | |
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MedLine Citation:
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PMID: 7552351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transferrin receptors are present in the plasma membrane of brain endothelial cells but it is unclear if these receptors mediate transport of transferrin across the blood-brain barrier (BBB). In the present study, the transport of rat holo-transferrin (rTf) across the BBB in vivo was evaluated in ketamine anesthetized rats (250-300 g) both by in situ brain perfusion coupled with capillary depletion analysis and by thaw-mount autoradiography. [125I]rTf was infused through the right internal carotid artery at a rate of 3.65 ml/min for 2.5-5 min. After a 5 min perfusion, the volume of distribution (VD) of [125I]rTf in the brain homogenate, the postvascular supernatant, or the vascular pellet was 55.8 +/- 4.5, 43.5 +/- 4.8, and 8.7 +/- 2.3 microliters/g, respectively. Co-infusion of [125I]rTf with unlabeled rTf or with a high dose of OX26 monoclonal antibody to the rat transferrin receptor significantly reduced the [125I]rTf transport, and in the presence of 10% rat serum [125I]rTf transport was nearly entirely abolished. The transport of [125I]rTf across the BBB in vivo was demonstrated by thaw-mount autoradiography, which showed silver grains well within brain parenchyma following a 5 min internal carotid artery perfusion. These studies are consistent with the following conclusions: (a) in the absence of competing plasma transferrin, [125I]holo-transferrin is transported through the BBB at a rate comparable to the OX26 monoclonal antibody; and (b) the ability to detect measurable transport of perfused [125I]transferrin is greatly inhibited by a small contamination of the perfusate by rat serum, which contains high concentrations of competing transferrin. |
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Authors:
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S Skarlatos; T Yoshikawa; W M Pardridge |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 683 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-11-21 Completed Date: 1995-11-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 164-71 Citation Subset: IM |
Affiliation:
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Department of Medicine, UCLA School of Medicine 90024, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Autoradiography Biological Transport / immunology, physiology Blood Proteins / pharmacology Blood-Brain Barrier / immunology, physiology* Capillaries / metabolism Carotid Arteries / metabolism Iodine Radioisotopes / diagnostic use Male Perfusion Rats Rats, Sprague-Dawley Regional Blood Flow Transferrin / pharmacokinetics* Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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R01-AI28760/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Blood Proteins; 0/Iodine Radioisotopes; 10028-17-8/Tritium; 11096-37-0/Transferrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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