Document Detail


Transplantation of human umbilical cord blood or amniotic epithelial stem cells alleviates mechanical allodynia after spinal cord injury in rats.
MedLine Citation:
PMID:  23294734     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Stem cell therapy is a potential treatment for spinal cord injury (SCI) and a variety of different stem cell types have been grafted into humans suffering from spinal cord trauma or into animal models of spinal injury. Although several studies have reported functional motor improvement after transplantation of stem cells into injured spinal cord, the benefit of these cells for treating SCI-induced neuropathic pain is not clear. In this study, we investigated the therapeutic effect of transplanting human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) or amniotic epithelial stem cells (hAESCs) on SCI-induced mechanical allodynia (MA) and thermal hyperalgesia (TH) in T13 spinal cord hemisected rats. Two weeks after SCI, hUCB-MSCs or hAESCs were transplanted around the spinal cord lesion site and behavioral tests were performed to evaluate changes in SCI-induced MA and TH. Immunohistochemical and western blot analyses were also performed to evaluate possible therapeutic effects on SCI-induced inflammation and the nociceptive related phosphorylation of the NMDA NR1 receptor subunit. While transplantation of hUCB-MSCs showed a tendency to reduce MA, transplantation of hAESCs significantly reduced MA. Neither hUCBMSCs nor hAESCs transplantation had any effect on SCI-induced TH. Transplantation of hAESCs also significantly reduced the SCI-induced increase in NMDA receptor NR1 subunit phosphorylation (pNR1) expression in the spinal cord. Both hUCB-MSCs and hAESCs reduced the SCI-induced increase in spinal cord expression of the microglial marker, F4/80, but not the increased expression of GFAP or iNOS. Taken together these findings demonstrate that the transplantation of hAESCs into the injured spinal cord can suppress mechanical allodynia and this effect seems to be closely associated with the modulation of spinal cord microglia activity and NR1 phosphorylation.
Authors:
Dae-Hyun Roh; Min-Soo Seo; Hoon-Seong Choi; Sang-Bum Park; Ho-Jae Han; Alvin J Beitz; Kyung-Sun Kang; Jang-Hern Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  Cell transplantation     Volume:  -     ISSN:  1555-3892     ISO Abbreviation:  Cell Transplant     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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