Document Detail


Transplantation of human fetal tissue for neurodegenerative diseases : Validation of a new protocol for microbiological analysis and bacterial decontamination.
MedLine Citation:
PMID:  23635602     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Restorative cell therapy concepts in neurodegenerative diseases are aimed at replacing lostneurons. Despite advances in research on pluripotent stem cells, fetal tissue from routineelective abortions is still regarded as the only safe cell source. Progenitor cells isolated fromdistinct first-trimester fetal CNS regions have already been used in clinical trials, and will beused again in a new multicenter trial funded by the European Union (TRANSEURO).Bacterial contamination of human fetal tissue poses a potential risk of causing infections inthe brain of the recipient. Thus, effective methods of microbial decontamination andvalidation of these methods are required prior to approval of a neurorestorative cell therapytrial. We have developed a protocol consisting of subsequent washing steps at differentstages of tissue processing. Efficacy of microbial decontamination was assessed on ratembryonic tissue incubated with high concentrations of defined microbe solutions includingrepresentative bacterial and fungal species. Experimental microbial contamination wasreduced by several log ranks. Subsequently, we have analyzed the spectrum of microbialcontamination and the effect of subsequent washing steps on aborted human fetal tissue.47.7% of the samples taken during human fetal tissue processing were positive for amicrobial contamination. After washing, no sample exhibited bacterial growth. Our datasuggest that human fetal tissue for neural repair can carry microbes of various species,highlighting the need for decontamination procedures. The decontamination protocoldescribed in this report has been shown to be effective as no microbes could be detected atthe end of the procedure.
Authors:
Tobias Piroth; Marie Christin Pauly; Christian Schneider; Annette Wittmer; Sven Mollers; Mate Dobrossy; Christian Winkler; Guido Nikkhah
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-29
Journal Detail:
Title:  Cell transplantation     Volume:  -     ISSN:  1555-3892     ISO Abbreviation:  Cell Transplant     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-5-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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