| Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. | |
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MedLine Citation:
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PMID: 22280565 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: The aim of this study was to investigate whether cells originating from human embryonic stem cells (hESCs) could be successfully transplanted onto a partially wounded human cornea. A second aim was to study the ability of the transplanted cells to differentiate into corneal epithelial-like cells. Methods: Spontaneously, differentiated hESCs were transplanted onto a human corneal button (without limbus) with the epithelial layer partially removed. The cells were cultured on Bowman's membrane for up to 9 days, and the culture dynamics documented in a time-lapse system. As the transplanted cells originated from a genetically engineered hESC line, they all expressed green fluorescent protein, which facilitated their identification during the culture experiments, tissue preparation and analysis. To detect any differentiation into human corneal epithelial-like cells, we analysed the transplanted cells by immunohistochemistry using antibodies specific for CK3, CK15 and PAX6. Results: The transplanted cells established and expanded on Bowman's membrane, forming a 1-4 cell layer surrounded by host corneal epithelial cells. Expression of the corneal marker PAX6 appeared 3 days after transplantation, and after 6 days, the cells were expressing both PAX6 and CK3. Conclusion: This shows that it is possible to transplant cells originating from hESCs onto Bowman's membrane with the epithelial layer partially removed and to get these cells to establish, grow and differentiate into corneal epithelial-like cells in vitro. |
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Authors:
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Charles Hanson; Thorir Hardarson; Catharina Ellerström; Markus Nordberg; Gunilla Caisander; Mahendra Rao; Johan Hyllner; Ulf Stenevi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-26 |
Journal Detail:
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Title: Acta ophthalmologica Volume: - ISSN: 1755-3768 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101468102 Medline TA: Acta Ophthalmol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 The Authors. Acta Ophthalmologica © 2012 Acta Ophthalmologica Scandinavica Foundation. |
Affiliation:
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Unit of Clinical Sciences, Department of Obstetrics and Gynecology, Sahlgrenska University Hospital, University of Gothenburg, Göteborg, Sweden Fertilitetscentrum, Carlanderska Hospital, Gothenburg, Sweden Cellartis AB, Göteborg, Sweden Life Technologies, Frederick, MD, USA Department of Ophthalmology, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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