Document Detail

Transplantation of gene-modified human bone marrow stromal cells into mouse-human bone chimeras.
MedLine Citation:
PMID:  10813530     Owner:  NLM     Status:  MEDLINE    
Transplantation of BM stromal cells engineered to secrete therapeutic factors could represent a treatment for a large array of hematologic disorders. The aim of this study was to evaluate the susceptibility of human BM stromal cell precursors to retroviral gene transfer, then the ability of those to be transplanted in vivo. We have transduced a recombinant retrovirus encoding the mouse CD2 antigen into STRO-1+ cells selected from adult and fetal BM. Gene-modified stromal cells were injected intravenously into NOD-SCID mice engrafted previously with pieces of human fetal hematopoietic bone. Using nested PCR, transgenic human cells were detected both in the marrow of human bone grafts and in the BM, liver, and spleen of host mice 7 weeks after grafting. These data indicate that BM stromal progenitor cells are targets for retrovirus-mediated gene transfer and can home to hematopoietic tissues on engraftment through the bloodstream of nonconditioned hosts.
N Brouard; A Chapel; D Thierry; P Charbord; B Péault
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of hematotherapy & stem cell research     Volume:  9     ISSN:  1525-8165     ISO Abbreviation:  J. Hematother. Stem Cell Res.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-08-21     Completed Date:  2000-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892915     Medline TA:  J Hematother Stem Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Institut de Protection et de Sûreté Nucléaire, Département de Protection de la Santé de l'Homme et de Dosimétrie, Fontenay-aux-Roses, France.
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MeSH Terms
Bone Marrow Cells / immunology
Bone Marrow Transplantation*
Bone Transplantation / methods
Cell Culture Techniques
Fetal Tissue Transplantation / methods
Gene Therapy / methods*
Mice, Inbred NOD
Mice, SCID
Stromal Cells / immunology,  transplantation
Tissue Distribution
Transplantation Chimera
Transplantation, Heterologous*

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