Document Detail


Transplantation of frozen-thawed late-gestational-age human fetal ovaries into immunodeficient mice.
MedLine Citation:
PMID:  18692825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare the development of human fetal follicles from late-gestational-age fetuses frozen-thawed gradually and slowly with dimethylsulfoxide (DMSO) or 1,2 propanediol (PROH) and sucrose after renal grafting into follicle stimulating hormone-treated immunodeficient mice. DESIGN: Controlled histologic study of grafted human fetal ovaries. SETTING: Major tertiary care academic center. PATIENT(S): Eleven women undergoing pregnancy termination at 22 to 33 gestational weeks. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Microscopic morphometric analysis and immunohistochemistry for proliferating cell nuclear antigen (PCNA). RESULT(S): Only follicles from samples frozen-thawed with PROH developed to secondary and antral stages 4 to 6 months after grafting, with PCNA expression in their granulosa cells. However, the number of surviving/developing follicles per section was very low (4-25 per graft), compared with 71 to 406 follicles in pretransplantation samples. Graft recovery was very high, with similar rates for transplants frozen-thawed with PROH and DMSO. Normal ovarian structure after grafting was identified only in the PROH frozen-thawed grafts. In deteriorated grafts, frozen-thawed with either DMSO or PROH, net-like hollows replaced follicles, whereas tubule-like structures were only identified in DMSO frozen-thawed grafts. CONCLUSION(S): This is the first report of the development of late-pregnancy-stage human fetal follicles in immunodeficient mice. PROH freezing-thawing supported development and survival better than DMSO. However, the low follicular survival points to the urgent need for efficient methods to enhance vascularization rate and prevent ischemia.
Authors:
Ronit Abir; Tal Biron-Shental; Raoul Orvieto; Roni Garor; Haim Krissi; Benjamin Fisch
Related Documents :
2521865 - Influence of different stimulation treatments on oocyte characteristics and in-vitro fe...
8981125 - Oocyte shortage for donation may be overcome in a programme with anonymous permutation ...
1472815 - In vitro fertilization and gamete micromanipulation in the lowland gorilla.
11784035 - Microtubule patterning during meiotic maturation in mouse oocytes is determined by cell...
6636465 - Effects of hemophilus somnus on the pregnant bovine reproductive tract and conceptus fo...
1443065 - Temperament and adjustment in young adulthood: a 15-year longitudinal analysis.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-09
Journal Detail:
Title:  Fertility and sterility     Volume:  92     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  770-7     Citation Subset:  IM    
Affiliation:
Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cryopreservation / methods*
Female
Fetus / cytology*
Humans
Mice
Mice, Inbred C57BL
Mice, Nude
Mice, SCID
Ovary / embryology*,  transplantation*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activating transcription factor 3 gene expression suggests that tissue stress plays a role in leiomy...
Next Document:  A comparison of combined laparoscopic uterine artery ligation and myomectomy versus laparoscopic myo...