| Transplantation of frozen-thawed late-gestational-age human fetal ovaries into immunodeficient mice. | |
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MedLine Citation:
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PMID: 18692825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To compare the development of human fetal follicles from late-gestational-age fetuses frozen-thawed gradually and slowly with dimethylsulfoxide (DMSO) or 1,2 propanediol (PROH) and sucrose after renal grafting into follicle stimulating hormone-treated immunodeficient mice. DESIGN: Controlled histologic study of grafted human fetal ovaries. SETTING: Major tertiary care academic center. PATIENT(S): Eleven women undergoing pregnancy termination at 22 to 33 gestational weeks. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Microscopic morphometric analysis and immunohistochemistry for proliferating cell nuclear antigen (PCNA). RESULT(S): Only follicles from samples frozen-thawed with PROH developed to secondary and antral stages 4 to 6 months after grafting, with PCNA expression in their granulosa cells. However, the number of surviving/developing follicles per section was very low (4-25 per graft), compared with 71 to 406 follicles in pretransplantation samples. Graft recovery was very high, with similar rates for transplants frozen-thawed with PROH and DMSO. Normal ovarian structure after grafting was identified only in the PROH frozen-thawed grafts. In deteriorated grafts, frozen-thawed with either DMSO or PROH, net-like hollows replaced follicles, whereas tubule-like structures were only identified in DMSO frozen-thawed grafts. CONCLUSION(S): This is the first report of the development of late-pregnancy-stage human fetal follicles in immunodeficient mice. PROH freezing-thawing supported development and survival better than DMSO. However, the low follicular survival points to the urgent need for efficient methods to enhance vascularization rate and prevent ischemia. |
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Authors:
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Ronit Abir; Tal Biron-Shental; Raoul Orvieto; Roni Garor; Haim Krissi; Benjamin Fisch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-09 |
Journal Detail:
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Title: Fertility and sterility Volume: 92 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 770-7 Citation Subset: IM |
Affiliation:
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Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cryopreservation / methods* Female Fetus / cytology* Humans Mice Mice, Inbred C57BL Mice, Nude Mice, SCID Ovary / embryology*, transplantation* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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