| Transplantation of bone mesenchymal stem cells containing the nerve growth factor gene in adult rat brain with Fimbria Fornix lesion. | |
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MedLine Citation:
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PMID: 19401170 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bone marrow-derived mesenchymal stem cells (BMSCs) are a promising source for cell-based treatment of brain injury, but the therapy of BMSCs is restricted by low cell survival. We examined whether nerve growth factor (NGF) improve BMSCs viability in the brain with Fimbria-Fornix lesion (FF). After transduction of NGF gene via recombinant retroviral vectors, the rat BMSCs were transformed into the NGF-GFP positive BMSCs, nearly 100% of cells expressed NGF. After transplanted into basal forebrain of rat with FF, the NGF-GFP positive BMSCs expressed the exogenous NGF gene in the host brain, and interesting, the survival number of BMSCs in the NGF group was significant more than that of the void plasmid group. Furthermore, the number of choline acetyltransferase (ChAT) immunoreactive neurons of NGF group was also significant higher than those of the void plasmid group (p<0.05) or the PBS group (p<0.01). Performance in the water maze test was improved in these rats in NGF group. These results indicate that NGF increased BMSCs survival in brain with FF, which results in better improvement of brain function than injected with BMSCs alone. |
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Authors:
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Shui-Long Leng; Wen-Guo Fan; Huai-Gang Qu; Shi-Cheng Xu; Wan-Ying Zhong; Wen-Yong Zhang; Jing-Quan Zeng; Da-Hong Long; Hong-Wen He |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2009-1-19 |
Journal Detail:
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Title: Brain research Volume: - ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-4-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Human Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, PR China.; Department of Human Anatomy, Guangzhou Medical College, Guangzhou, Guangdong 510182, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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