Document Detail


Transplantation of adrenal cortical progenitor cells enriched by Nile red.
MedLine Citation:
PMID:  19592014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The adrenal cortex may contain progenitor cells useful for tissue regeneration. Currently there are no established methods to isolate these cells.
MATERIAL AND METHODS: Murine adrenal cells were sorted into a Nile-red-bright (NR(bright)) and a Nile-red-dim (NR(dim)) population of cells according to their degree of cholesterol content revealed by Nile red fluorescence. The cells were transplanted under the renal capsule to determine their ability for regeneration.
RESULTS: The NR(bright) cells contained an abundance of lipid droplets, whereas the NR(dim) cells contained little. The NR(bright) cells expressed Sf1 and the more differentiated adrenal cortical genes, including Cyp11a1, Cyp11b1, and Cyp11b2, whereas the NR(dim) cells expressed Sf1 but not the more differentiated adrenal cortical genes. After 56 d of implantation in unilateral adrenalectomized mice, the NR(dim) cells expressed Sf1 and the more differentiated adrenal cortical genes, whereas the NR(bright) cells ceased to express Sf1 as well as the more differentiated adrenal cortical genes. NR(dim) cells also proliferated in the presence of basic fibroblast growth factor.
CONCLUSIONS: The population of NR(dim) cells contained adrenal cortical progenitor cells that can proliferate and give rise to differentiated daughter cells. These cells may be useful for adrenal cortical regeneration.
Authors:
James C Y Dunn; Yinting Chu; Harry H Qin; Tatiana Zupekan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-05-14
Journal Detail:
Title:  The Journal of surgical research     Volume:  156     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-23     Completed Date:  2009-09-25     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-24     Citation Subset:  IM    
Affiliation:
Department of Surgery and Department of Bioengineering, Division of Pediatric Surgery, Biomedical Engineering Interdepartmental Program, University of California, Los Angeles, California 90095-7098, USA. jdunn@mednet.ucla.edu
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex / cytology*
Animals
Cell Culture Techniques / methods*
Fluorescent Dyes*
Male
Mice
Mice, Inbred C57BL
Models, Animal
Oxazines*
Regeneration*
Stem Cell Transplantation*
Stem Cells / physiology*
Grant Support
ID/Acronym/Agency:
DK068207/DK/NIDDK NIH HHS; K08 DK068207/DK/NIDDK NIH HHS; K08 DK068207-04/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oxazines; P476F1L81G/nile red
Comments/Corrections
Comment In:
J Surg Res. 2010 Jun 1;161(1):34-5   [PMID:  20031173 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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