Document Detail

Transplantation of adrenal cortical progenitor cells enriched by Nile red.
MedLine Citation:
PMID:  19592014     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The adrenal cortex may contain progenitor cells useful for tissue regeneration. Currently there are no established methods to isolate these cells.
MATERIAL AND METHODS: Murine adrenal cells were sorted into a Nile-red-bright (NR(bright)) and a Nile-red-dim (NR(dim)) population of cells according to their degree of cholesterol content revealed by Nile red fluorescence. The cells were transplanted under the renal capsule to determine their ability for regeneration.
RESULTS: The NR(bright) cells contained an abundance of lipid droplets, whereas the NR(dim) cells contained little. The NR(bright) cells expressed Sf1 and the more differentiated adrenal cortical genes, including Cyp11a1, Cyp11b1, and Cyp11b2, whereas the NR(dim) cells expressed Sf1 but not the more differentiated adrenal cortical genes. After 56 d of implantation in unilateral adrenalectomized mice, the NR(dim) cells expressed Sf1 and the more differentiated adrenal cortical genes, whereas the NR(bright) cells ceased to express Sf1 as well as the more differentiated adrenal cortical genes. NR(dim) cells also proliferated in the presence of basic fibroblast growth factor.
CONCLUSIONS: The population of NR(dim) cells contained adrenal cortical progenitor cells that can proliferate and give rise to differentiated daughter cells. These cells may be useful for adrenal cortical regeneration.
James C Y Dunn; Yinting Chu; Harry H Qin; Tatiana Zupekan
Related Documents :
19588164 - Involvement of centrosomes in nuclear irregularity of thyroid carcinoma cells.
8787964 - Immunocytochemical characterization of two thyroid medullary carcinoma cell lines in vi...
3428884 - Signet ring cell 'mucinous' thyroid adenoma: a follicle cell tumour with abnormal accum...
10705394 - Salivary gland basal cell and canalicular adenomas: immunohistochemical demonstration o...
10774924 - Nuclear localization of metallothionein during cell proliferation and differentiation.
15057964 - Monitoring of apoptosis in intact single cells with photothermal microscope.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-05-14
Journal Detail:
Title:  The Journal of surgical research     Volume:  156     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-23     Completed Date:  2009-09-25     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-24     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adrenal Cortex / cytology*
Cell Culture Techniques / methods*
Fluorescent Dyes*
Mice, Inbred C57BL
Models, Animal
Stem Cell Transplantation*
Stem Cells / physiology*
Grant Support
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oxazines; P476F1L81G/nile red
Comment In:
J Surg Res. 2010 Jun 1;161(1):34-5   [PMID:  20031173 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dexamethasone alters the hepatic inflammatory cellular profile without changes in matrix degradation...
Next Document:  Development of quality indicators of care for patients undergoing hepatic resection for metastatic c...