Document Detail


Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.
MedLine Citation:
PMID:  21336180     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this study was to track and investigate the effects of autologous bone marrow-derived mesenchymal stem cells (MSCs) transplantation after acute myocardial infarction in swine assessed by magnetic resonance imaging (MRI).
MATERIALS AND METHODS: Twenty-four Chinese mini-pigs (27±3 kg) were divided into 4 groups, including control groups (groups 1 and 3) and MSCs transplantation groups (group 2, super paramagnetic iron oxide labeled and group 4, 4',6-diamidino-2-phenylindole labeled). Super paramagnetic iron oxide-labeled and 4',6-diamidino-2-phenylindole-labeled MSCs (3.0×10⁶ cells/mL) with a volume of 10 mL were injected into the left anterior descending artery by a catheter at 1 week after acute myocardial infarction, respectively. Cell distribution, cardiac functions, and scar tissue were quantitatively assessed by MRI.
RESULTS: The reduction of the T2* value in the myocardium, spleen, and liver in group 2 was significantly greater than that in group 1. MRI showed that function and scar size at baseline and 3 days after cell infusion were not significantly different between groups 1 and 2. Six weeks later left ventricular ejection fraction (P<0.0001), end-systolic volume (P<0.05), the number of dyskinetic segments (P<0.0001), left ventricular weight index (P<0.0001), and the infarcted size (P<0.0001) in group 4 were all improved comparing with those in group 3.
CONCLUSIONS: The majority of MSCs entrapped by the extracardial organs were mainly in the spleen. Catheter-based delivery of autologous bone marrow-derived MSCs into infarcted myocardium is feasible and effective.
Authors:
Minjie Lu; Shihua Zhao; Qiong Liu; Shiliang Jiang; Peng Song; Haiyan Qian; Yan Zhang; Jian Ling; Chaowu Yan; Huaibing Cheng; Ning Ma; Hong Zhao; Yuqing Liu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of thoracic imaging     Volume:  27     ISSN:  1536-0237     ISO Abbreviation:  J Thorac Imaging     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-06-19     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  8606160     Medline TA:  J Thorac Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-35     Citation Subset:  IM    
Affiliation:
Department of Radiology, Fuwai Hospital, Peking Union Medical College, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Dextrans / pharmacology
Immunohistochemistry
Indoles / pharmacology
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles
Mesenchymal Stem Cell Transplantation*
Microscopy, Confocal
Myocardial Infarction / therapy*
Polymerase Chain Reaction
Random Allocation
Spleen / cytology
Statistics, Nonparametric
Swine
Swine, Miniature
Transplantation, Autologous
Chemical
Reg. No./Substance:
0/Indoles; 0/Magnetite Nanoparticles; 47165-04-8/DAPI; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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