Document Detail

Transplantation of Side Population Cells Restores the Function of Damaged Exocrine Glands Through Clusterin.
MedLine Citation:
PMID:  22782911     Owner:  NLM     Status:  Publisher    
Stem cell-based therapy has been proposed as a promising strategy for regenerating tissues lost through incurable diseases. Side population (SP) cells have been identified as putative stem cells in various organs. To examine therapeutic potential of SP cells in hypofunction of exocrine glands, SP cells isolated from mouse exocrine glands, namely, lacrimal and salivary glands, were transplanted into mice with irradiation-induced hypofunction of the respective glands. The secretions from both glands in the recipient mice were restored within 2 months of transplantation, although the transplanted cells were only sparsely distributed and produced no outgrowths. Consistent with this, most SP cells were shown to be CD31-positive endothelial-like cells. In addition, we clarified that endothelial cell-derived clusterin, a secretory protein, was an essential factor for SP cell-mediated recovery of the hypofunctioning glands because SP cells isolated from salivary glands of clusterin-deficient mice had no therapeutic potential, whereas lentiviral transduction of clusterin restored the hypofunction. In vitro and in vivo studies showed that clusterin had an ability to directly inhibit oxidative stress and oxidative stress-induced cell damage. Thus, endothelial cell-derived clusterin possibly inhibit oxidative stress-induced hypofunction of these glands.
Kenji Mishima; Hiroko Inoue; Tatsuaki Nishiyama; Yo Mabuchi; Yusuke Amano; Fumio Ide; Makoto Matsui; Hiroyuki Yamada; Gou Yamamoto; Junichi Tanaka; Rika Yasuhara; Takashi Sakurai; Masaichi-Chang-Il Lee; Kan Chiba; Hidetoshi Sumimoto; Yutaka Kawakami; Yumi Matsuzaki; Kazuo Tsubota; Ichiro Saito
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-10
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  -     ISSN:  1549-4918     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 AlphaMed Press.
Department of Pathology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan; Department of Oral Pathology, Showa University School of Dentistry, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo, 145-8515, Japan.
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