| Transplantation of nonhematopoietic adult bone marrow stem/progenitor cells isolated by p75 nerve growth factor receptor into the penis rescues erectile function in a rat model of cavernous nerve injury. | |
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MedLine Citation:
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PMID: 20728109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Radical prostatectomy for prostate cancer frequently results in erectile dysfunction and decreased quality of life. We investigated the effects of transplanting nonhematopoietic adult bone marrow stem/progenitor cells (multipotent stromal cells) into the corpus cavernosum in a rat model of bilateral cavernous nerve crush injury. MATERIALS AND METHODS: Multipotent stromal cells were isolated from the bone marrow of transgenic green fluorescent protein rats by plastic adherence (rat multipotent stromal cells) or magnetic activated cell sorting using antibodies against p75 low affinity nerve growth factor receptor (p75 derived multipotent stromal cells). Bilateral cavernous nerve crush injury was induced in adult male Sprague-Dawley rats. Immediately after injury 8 rats each were injected intracavernously with phosphate buffered saline (vehicle control), fibroblasts (cell control), rat multipotent stromal cells (cell treatment) or p75 derived multipotent stromal cells (cell treatment). Another 8 rats underwent sham operation (phosphate buffered saline injection). Four weeks after the procedures we assessed erectile function by measuring the intracavernous-to-mean arterial pressure ratio and total intracavernous pressure during cavernous nerve stimulation. RESULTS: Intracavernous injection of p75 derived multipotent stromal cells after bilateral cavernous nerve crush injury resulted in a significantly higher mean intracavernous-to-mean arterial pressure ratio and total intracavernous pressure compared with all other groups except the sham operated group (p <0.05). Rats injected with typical multipotent stromal cells had partial erectile function rescue compared with animals that received p75 derived multipotent stromal cells. Fibroblast (cell control) and phosphate buffered saline (vehicle control) injection did not improve erectile function. Enzyme-linked immunosorbent assay suggested that basic fibroblast growth factor secreted by p75 derived multipotent stromal cells protected the cavernous nerve after bilateral cavernous nerve crush injury. CONCLUSIONS: Transplantation of adult stem/progenitor cells may provide an effective treatment for erectile dysfunction after radical prostatectomy. |
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Authors:
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Muammer Kendirci; Landon Trost; Benjamin Bakondi; Mandolin J Whitney; Wayne J G Hellstrom; Jeffrey L Spees |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-21 |
Journal Detail:
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Title: The Journal of urology Volume: 184 ISSN: 1527-3792 ISO Abbreviation: J. Urol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2010-10-04 Revised Date: 2012-04-27 |
Medline Journal Info:
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Nlm Unique ID: 0376374 Medline TA: J Urol Country: United States |
Other Details:
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Languages: eng Pagination: 1560-6 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2010 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Urology, Sisli Etfal Training and Research Hospital, Istanbul, Turkey. mkendirci@superonline.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Transplantation* Disease Models, Animal Erectile Dysfunction / etiology, surgery* Immunomagnetic Separation Male Multipotent Stem Cells / transplantation* Penis / injuries*, innervation* Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor Stem Cell Transplantation* |
| Grant Support | |
ID/Acronym/Agency:
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HL077570/HL/NHLBI NIH HHS; HL085210/HL/NHLBI NIH HHS; P20 RR016435/RR/NCRR NIH HHS; R01 HL085210-01A2/HL/NHLBI NIH HHS; R01 HL085210-02/HL/NHLBI NIH HHS; R01 HL085210-03/HL/NHLBI NIH HHS; R01 HL085210-04/HL/NHLBI NIH HHS; R21 HL077570-01/HL/NHLBI NIH HHS; R21 HL077570-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptor, Nerve Growth Factor |
| Comments/Corrections | |
Comment In:
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J Urol. 2011 Mar;185(3):1158-9; author reply 1159-61
[PMID:
21256512
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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