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Transplantation of liver organoids in the omentum and kidney.
MedLine Citation:
PMID:  20946288     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Liver organoids were reconstructed by mouse-immortalized hepatocytes and nonparenchymal cells (sinusoidal endothelial cells and hepatic stellate cells) in a radial-flow bioreactor (RFB). A biodegradable apatite-fiber scaffold (AFS) was used as a scaffold packed in the RFB, which enables three-dimensional cell cultures. The organoids cocultured in the RFB showed a liver-like structure with high-density layers of hepatocytes and the formation of vessel-like structures. A liver organoid consisting of three cocultured cells was transplanted under the kidney capsule (kidney group) or into the omentum (omentum group) using BALB/c nude mice. Transplanted liver organoids survived in the kidney or omentum. The expression of mRNAs of albumin, connexin 26 and 32, hepatocyte nuclear factor 4α, and glucose-6-phosphatase was increased in both groups at 8 weeks after transplantation in comparison to the pretransplant status. Tyrosine aminotransferase appeared only in the omentum group. The results suggested that the functions of liver organoids differed depending on the transplanted site in the recipient animals.
Authors:
Ryota Saito; Yuji Ishii; Ryusuke Ito; Keisuke Nagatsuma; Ken Tanaka; Masaya Saito; Haruka Maehashi; Hideki Nomoto; Kiyoshi Ohkawa; Hiroshi Mano; Mamoru Aizawa; Hiroshi Hano; Katsuhiko Yanaga; Tomokazu Matsuura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-14
Journal Detail:
Title:  Artificial organs     Volume:  35     ISSN:  1525-1594     ISO Abbreviation:  Artif Organs     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  80-3     Citation Subset:  IM    
Copyright Information:
© 2010, Copyright the Authors. Artificial Organs © 2010, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Affiliation:
Department of Surgery, Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
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