| Transplantation of CTLA4Ig gene-transduced adipose tissue-derived mesenchymal stem cells reduces inflammatory immune response and improves Th1/Th2 balance in experimental autoimmune thyroiditis. | |
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MedLine Citation:
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PMID: 21259404 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Autoimmune thyroiditis is one of common organ-specific autoimmune disease. The aim of this study was to observe the effect of adipose tissue derived mesenchymal stem cells (ATMSC) and CTLA4Ig gene-transduced ATMSC on autoimmune thyroiditis. METHODS: Experimental autoimmune thyroiditis was induced by immunization with thyroglobulin. Animals were divided into three groups: (i) a half million of human ATMSC, (ii) a half million of murine CLTA4Ig gene-transduced human ATMSC (CTLA4Ig-MSC), or (iii) normal saline (as control), which were administered intravenously four times within a 3-week period. Blood and tissue samples were collected 1 week after the last cell transplantation. RESULTS: The absorbance of serum thyroglobulin autoantibody (TgAA) in the CTLA4Ig-MSC group was considerably lower than those in other groups. In culture supernatant of LPS-stimulated spleen cells, both of the MSC-treated groups showed significantly lower absorbances of TgAA than the control. Flow cytometric analysis of spleen cells revealed a significant decrease in the proportion of CD3+ and CD11b in the CTLA4Ig-MSC group compared to the other groups. Lymphocyte infiltration in the thyroid glands was also dramatically decreased in both of MSC-treated groups. Cytokine analysis showed that ATMSC decreased the production of proinflammatory cytokines and improved the Th1/Th2 balance by down-regulating Th1 cytokines. CONCLUSION: Although CTLA4Ig-MSC transplantation had better result in reduction of serum TgAA, both of ATMSC and CTLA4Ig-MSC transplantations are promising treatments for autoimmune thyroiditis judging from the results of histopathology and cytokine analysis. They may be attractive candidates for treating organ-specific autoimmune disease. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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Eun Wha Choi; Il Seob Shin; Hee Woo Lee; So Young Park; Ji Hyun Park; Mi Hyun Nam; Jong Sung Kim; Sang Kyu Woo; Eun Ji Yoon; Sung Keun Kang; Jeong Chan Ra; Hwa Young Youn; Sung Hwa Hong |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of gene medicine Volume: 13 ISSN: 1521-2254 ISO Abbreviation: J Gene Med Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815764 Medline TA: J Gene Med Country: England |
Other Details:
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Languages: eng Pagination: 3-16 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory Animal Research Center, Samsung Biomedical Research Institute, 50 Irwon-dong, Gangnam-gu, Seoul 135-710, Republic of Korea. vet.cew@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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