Document Detail


Transplantation of BMSCs expressing hVEGF(165) /hBD3 promotes wound healing in rats with combined radiation-wound injury.
MedLine Citation:
PMID:  23137415     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The combined radiation-wound injury is a refractory wound with decreased number or dysfunction of repairing cells and growth factors. This remains a challenge in clinical practice. The object of this study is to evaluate the therapeutic efficacy of a combination of human vascular endothelial growth factor 165 (hVEGF(165) ) and human beta-defensin 3 (hBD3) in the treatment of such wound. A plasmid carrying hVEGF(165) gene and hBD3 gene was used to transfect rat bone marrow-derived mesenchymal stem cells (BMSCs). The supernatant from the modified BMSCs significantly promoted the proliferation and cell migration of human endothelial cells and it also inhibited the growth of bacteria and fungus, demonstrating the successful expression of the transfected genes. The hVEGF(165) /hBD3-modified BMSCs were then injected into the sites of combined radiation-wound injury on rats. It demonstrated that wound healing time was shortened significantly in the treated rats. The granulation tissue formation/maturation, skin appendage regeneration and collagen deposition were also improved significantly. Strong expression of hVEGF(165) and hBD3 was detected in the wound surface at early stage of the healing. The results indicate that topical transplantation of hVEGF(165) /hBD3-modified BMSCs promoted wound healing, and this gene therapy strategy presents a promising approach in the treatment of refractory wounds such as the combined radiation-wound injury.
Authors:
Zhangquan Xia; Congji Zhang; Yi Zeng; Tao Wang; Guoping Ai
Related Documents :
11375655 - Exchange of domain i from bacillus thuringiensis cry1 toxins influences protoxin stabil...
15231785 - The bacillus subtilis yqji gene encodes the nadp+-dependent 6-p-gluconate dehydrogenase...
20600285 - Suite of novel vectors for ectopic insertion of gfp, cfp and iyfp transcriptional fusio...
20717675 - Rpoe may promote flagellar gene expression in salmonella enterica serovar typhi under h...
18349385 - Transcriptional adaptation to clcn5 knockout in proximal tubules of mouse kidney.
23142035 - Non-neutral evolution in non-lee-encoded type iii effectors of attaching and effacing e...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  International wound journal     Volume:  -     ISSN:  1742-481X     ISO Abbreviation:  Int Wound J     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230907     Medline TA:  Int Wound J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. International Wound Journal © 2012 Blackwell Publishing Ltd and Medicalhelplines.com Inc.
Affiliation:
Z Xia, MM, Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing, China, Department of Stomatology, No 291 Hospital of the People's Liberation Army, Baotou, China C Zhang, MD, Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing, China Y Zeng, MD, Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing, China 3T Wang, MD, Department of Radiation Medicine, Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China G Ai, MD, Department of Radiation Medicine, Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sleep disorders in Machado-Joseph disease: A dopamine transporter imaging study.
Next Document:  The match between institutional elderly care management research and management challenges - a syste...