| Transplacental traffic after in utero mesenchymal stem cell transplantation. | |
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MedLine Citation:
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PMID: 20131967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transplacental traffic of fetal progenitor and differentiated cells is a well-known phenomenon in pregnancies. We hypothesize that intrauterine stem cell transplantation leads to microchimerism in the dams and that this is gestational age-dependent. EGFP+ fetal liver-derived mesenchymal stem cell (MSC) (10(5) per fetus) were injected intraperitoneally into congeneic and allogeneic recipient fetuses at E12 versus E13.5 of murine pregnancy (56 dams). Engraftment in maternal organs was evaluated using TaqMan quantitative polymerase chain reaction (PCR) and fluorescence microscopy during pregnancy (1, 3, and 7 days after in utero transplantation [IUT]) and after delivery (1 and 4 weeks after delivery). One day after IUT donor cells were mainly found in the placenta (E12: 9/10 dams vs. E13.5: 4/8 dams) and laparotomy site (E12: 5/10 dams vs. E13.5: 4/8 dams). Three days after IUT these probabilities decreased significantly in the placenta to 3/8 and 1/3, respectively, whereas it was increased within the surgical wound to 8/8 and 2/4. One week after IUT donor cells could be detected in other single maternal organs, such as bone marrow or spleen. The surgical wound was chimeric in all dams. One week after delivery the surgical wound was still a major site of engraftment in both groups. E12 IUT resulted in detectable donor cell microchimerism in the maternal bone marrow (3/4), liver (2/4), lungs (1/4), spleen (1/4), and thymus (1/4), whereas engraftment probabilities were lower following E13.5 IUT (BM: 1/4, liver: 2/4, lungs: 1/4, spleen: 1/4, thymus: 0/4). At 4 weeks after delivery persistent microchimerism was found only after E12 IUT in various maternal organs (BM: 1/4, spleen: 1/4, lungs: 1/4) and within newly created surgical wounds (3/4), but completely not in the E13.5 group. Allogeneic IUT did also not result in any detectable long-term fetal microchimerism. An earlier IUT might lead to a higher transplacental traffic of donor MSC and persistent microchimerism within maternal tissues. Even 4 weeks after delivery, these cells are present in surgical wounds. |
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Authors:
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Carolyn Troeger; Iryna Perahud; Stephan Moser; Wolfgang Holzgreve |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Stem cells and development Volume: 19 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
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Languages: eng Pagination: 1385-92 Citation Subset: IM |
Affiliation:
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Laboratory for Prenatal Medicine, Department of Obstetrics and Gynecology, University Hospital, Basel, Switzerland. ctroeger@uhbs.ch |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Female Fetoscopy / methods Fetus / surgery Graft Survival / physiology Maternal-Fetal Exchange / physiology* Mesenchymal Stem Cell Transplantation / methods* Mesenchymal Stem Cells / physiology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic Placenta / metabolism*, physiology, surgery Pregnancy Transcellular Cell Migration / physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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