| Transplacental transfer and biotransformation of genistein in human placenta. | |
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MedLine Citation:
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PMID: 20413155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To study transplacental transfer and biotransformation of genistein in the human placenta. STUDY DESIGN AND OUTCOMES: Human placentae obtained from healthy term singleton pregnancies were utilised in a dual re-circulating model of ex-vivo placental perfusion. Four placentae were perfused for 180min following addition of genistein (10ng/mL) to the maternal perfusate. Antipyrine and FITC dextran were used as positive and negative controls respectively to validate integrity of the circuits. Concentrations of genistein and its conjugates were determined by liquid chromatography-mass spectrometry (LC-MS). RESULTS: The transfer percentage for antipyrine and genistein was 25.6+/-1.40% and 22.1+/-1.61% respectively and the transfer index for genistein was 0.90+/-0.04 after 180min of perfusion. 12.0+/-2.40% of genistein in the fetal compartment and 7.36+/-4.73% of genistein in the maternal compartment were in the conjugated form. CONCLUSIONS: Genistein can transfer across the human placenta at environmentally relevant levels. Placental metabolizing enzymes conjugate a small fraction of genistein into the glucuronide/sulphate form, which is devoid of estrogenic action. |
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Authors:
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B Balakrishnan; E B Thorstensen; A P Ponnampalam; M D Mitchell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-22 |
Journal Detail:
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Title: Placenta Volume: 31 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-08-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 506-11 Citation Subset: IM |
Affiliation:
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The Liggins Institute, The University of Auckland, Grafton, Auckland, New Zealand. b.balakrishnan@auckland.ac.nz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotransformation Chromatography, High Pressure Liquid Female Genistein / pharmacokinetics* Humans Maternal-Fetal Exchange / drug effects* Phytoestrogens / pharmacokinetics Placenta / drug effects* Pregnancy Tandem Mass Spectrometry |
| Chemical | |
Reg. No./Substance:
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0/Phytoestrogens; 446-72-0/Genistein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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