| Transmural stretch-dependent regulation of contractile properties in rat heart and its alteration after myocardial infarction. | |
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MedLine Citation:
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PMID: 15498894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The "stretch-sensitization" response is essential to the regulation of heart contractility. An increase in diastolic volume improves systolic contraction. The cellular mechanisms of this modulation, the Frank-Starling law, are still uncertain. Moreover, their alterations in heart failure remains controversial. Here, using left ventricular skinned rat myocytes, we show a nonuniform stretch-sensitization of myofilament activation across the ventricular wall. Stretch-dependent Ca2+ sensitization of myofilaments increases from sub-epicardium to sub-endocardium and is correlated with an increase in passive tension. This passive tension-dependent component of myofibrillar activation is not associated with expression of titin isoforms, changes in troponin I level, and phosphorylation status. Instead, we observe that stretch induces phosphorylation of ventricular myosin light chain 2 isoform (VLC2b) in sub-endocardium specifically. Thus, VLC2b phosphorylation could act as a stretch-dependent modulator of activation tuned within normal heart. Moreover, in postmyocardial infarcted rat, the gradient of stretch-dependent Ca2+ sensitization disappears associated with a lack of VLC2b phosphorylation in sub-endocardium. In conclusion, nonuniformity is a major characteristic of the normal adult left ventricle (LV). The heterogeneous myocardial deformation pattern might be caused not only by the morphological heterogeneity of the tissue in the LV wall, but also by the nonuniform contractile properties of the myocytes across the wall. The loss of a contractile transmural gradient after myocardial infarction should contribute to the impaired LV function. |
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Authors:
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Olivier Cazorla; Szabolcs Szilagyi; Jean-Yves Le Guennec; Guy Vassort; Alain Lacampagne |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-10-21 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 19 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-04 Completed Date: 2005-11-07 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 88-90 Citation Subset: IM |
Affiliation:
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INSERM U-637, CHU Arnaud de Villeneuve, Montpellier, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiac Myosins / metabolism Contractile Proteins / metabolism* Heart* Heart Ventricles / cytology, metabolism Male Muscle Proteins / metabolism Myocardial Infarction / metabolism* Myocardium / metabolism Myocytes, Cardiac / metabolism Myosin Light Chains / metabolism Phosphorylation Protein Isoforms / metabolism Rats Rats, Wistar Ventricular Myosins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Contractile Proteins; 0/Muscle Proteins; 0/Myosin Light Chains; 0/Protein Isoforms; 0/myosin light chain 2; EC 3.6.1.-/Cardiac Myosins; EC 3.6.1.-/Ventricular Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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