Document Detail


Transmission of intestinal Bifidobacterium longum subsp. longum strains from mother to infant, determined by multilocus sequencing typing and amplified fragment length polymorphism.
MedLine Citation:
PMID:  21821739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gastrointestinal tracts of neonates are colonized by bacteria immediately after birth. It has been discussed that the intestinal microbiota of neonates includes strains transferred from the mothers. Although some studies have indicated possible bacterial transfer from the mother to the newborn, this is the first report confirming the transfer of bifidobacteria at the strain level. Here, we investigated the mother-to-infant transmission of Bifidobacterium longum subsp. longum by genotyping bacterial isolates from the feces of mothers before delivery and of their infants after delivery. Two hundred seven isolates from 8 pairs of mothers and infants were discriminated by multilocus sequencing typing (MLST) and amplified fragment length polymorphism (AFLP) analysis. By both methods, 11 strains of B. longum subsp. longum were found to be monophyletic for the feces of the mother and her infant. This finding confirms that these strains were transferred from the intestine of the mother to that of the infant. These strains were found in the first feces (meconium) of the infant and in the feces at days 3, 7, 30, and 90 after birth, indicating that they stably colonize the infant's intestine immediately after birth. The strains isolated from each family did not belong to clusters derived from any of the other families, suggesting that each mother-infant pair might have unique family-specific strains.
Authors:
Hiroshi Makino; Akira Kushiro; Eiji Ishikawa; Delphine Muylaert; Hiroyuki Kubota; Takafumi Sakai; Kenji Oishi; Rocio Martin; Kaouther Ben Amor; Raish Oozeer; Jan Knol; Ryuichiro Tanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-05
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-23     Completed Date:  2012-01-19     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6788-93     Citation Subset:  IM    
Affiliation:
Yakult Honsha European Research Center for Microbiology, ESV, Technologiepark 4, 9052 Ghent-Zwijnaarde, Belgium. hiroshi.makino@yher.be
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MeSH Terms
Descriptor/Qualifier:
Amplified Fragment Length Polymorphism Analysis / methods
Bifidobacteriales Infections / microbiology,  transmission*
Bifidobacterium / classification*,  genetics*,  isolation & purification
Carrier State / microbiology,  transmission*
Cluster Analysis
Feces / microbiology
Genotype
Humans
Infant
Molecular Typing / methods*
Multilocus Sequence Typing / methods
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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