Document Detail

Translocation of coagulase-negative bacterial staphylococci in rats following intestinal ischemia-reperfusion injury.
MedLine Citation:
PMID:  14646338     Owner:  NLM     Status:  MEDLINE    
Many patients with sepsis have bacteremia for which no septic focus is identified either clinically or by autopsy. This study was designed to determine the relationship between the ischemia-reperfusion injury (IRI) and bacterial translocation that might be involved in the pathogenesis of necrotizing enterocolitis. In the first experiment, a total of 32 Sprague-Dawley rats weighing 150-200 g were divided into four groups. The mesentery to isolated loop was occluded for 30, 60, and 90 min following 30-min reperfusion in the three groups of experimental animals with a micro-bulldog clamp. A control group involved the same technique and exposure, without occlusion of the mesentery. Two sets of blood culture were taken through a catheter in the portal vein immediately and 15 min after the reperfusion, respectively. In another experiment, bacteria isolated were fed in different doses to control rats and those after 30- or 60-min ischemia and 30-min reperfusion. Two sets of blood culture were taken following the procedure. Invasion and transcytosis of the bacteria through epithelial cells were studied in vitro using a Madin-Derby canine kidney (MDCK) cell monolayer model. PCR for delta toxin gene was performed on all bacteria isolated, using Staphylococcus epidermidis as the control. Coagulase-negative staphylococci (CoNS) were invariably isolated from mice with prolonged ischemia (90 min) and reperfusion. When bacteria were fed into mice with only 30-min ischemia, an inoculum as low as 5 x 10(5) CFU/ml could induce bacteremia. No bacterial translocation was found in control mice even fed with a higher dose of bacteria (5 x 10(8) CFU/ml). In vitro experiments showed that CoNS failed to transcytose MDCK monolayer. These isolates were not cytotoxic to MDCK cells and contained no delta toxin gene. Bacterial translocation of CoNS occurred following severe bowel ischemia and reperfusion injury. Intact mucosa integrity readily prevented bacterial translocation; however, bacterial translocation could occur in rats following mild IRI in the presence of a higher number of CoNS in the gut.
Chih-Cheng Luo; Hsiang-Hung Shih; Cheng-Hsun Chiu; Jer-Nan Lin
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Publication Detail:
Type:  Journal Article     Date:  2003-11-25
Journal Detail:
Title:  Biology of the neonate     Volume:  85     ISSN:  0006-3126     ISO Abbreviation:  Biol. Neonate     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0247551     Medline TA:  Biol Neonate     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  151-4     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Departments of Pediatric Surgery and Pediatrics, Chang Gung Children's Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan.
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MeSH Terms
Bacterial Translocation / physiology*
Coagulase / deficiency
Colony Count, Microbial
Intestinal Diseases / microbiology*,  pathology
Intestinal Mucosa / microbiology,  pathology
Intestines / blood supply
L-Lactate Dehydrogenase / metabolism
Rats, Sprague-Dawley
Reperfusion Injury / microbiology*,  pathology
Sepsis / microbiology,  pathology
Staphylococcal Infections / microbiology*,  pathology
Staphylococcus / enzymology,  physiology*
Reg. No./Substance:
0/Coagulase; EC Dehydrogenase

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