Document Detail


Translational regulation by steroids. Identification of a steroid modulatory element in the 5'-untranslated region of the myelin basic protein messenger RNA.
MedLine Citation:
PMID:  1700788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although steroids have been implicated in post-transcriptional regulation, their effects on mRNA translation rates have been uncertain. We have used a cell-free translation system programmed with synthetic messages to show that steroids can alter the translation rates of a number of myelin protein mRNAs and the mRNA encoding a non-myelin protein, the estrogen receptor. Through the use of deletion analysis, site-directed mutagenesis, and chimeric mRNAs we have identified a 9-nucleotide segment in the 5'-untranslated region of one myelin protein mRNA that is necessary for steroid action. Steroid-mediated translational regulation is discussed in terms of myelination where subtle developmental changes in protein composition of the membrane have significant consequences on its morphology and function. We propose that the modulation of mRNA translation rates by steroids is a more general phenomenon that may serve as another mechanism by which steroids can regulate gene expression.
Authors:
J M Verdi; A T Campagnoni
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  265     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-28     Completed Date:  1990-12-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  20314-20     Citation Subset:  IM    
Affiliation:
Mental Retardation Research Center, UCLA School of Medicine 90024.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell-Free System
Cloning, Molecular
Estradiol / pharmacology*
Hydrocortisone / pharmacology*
Kinetics
Methionine / metabolism
Molecular Sequence Data
Mutagenesis, Site-Directed
Myelin Basic Proteins / biosynthesis,  genetics*
Oligonucleotide Probes
Polymerase Chain Reaction
Protein Biosynthesis / drug effects*
RNA, Messenger / drug effects,  genetics*
Rabbits
Restriction Mapping
Reticulocytes / metabolism
Transcription, Genetic
Tyrosine 3-Monooxygenase / genetics
Grant Support
ID/Acronym/Agency:
NS 23022/NS/NINDS NIH HHS; NS 23322/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Myelin Basic Proteins; 0/Oligonucleotide Probes; 0/RNA, Messenger; 50-23-7/Hydrocortisone; 50-28-2/Estradiol; 63-68-3/Methionine; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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