Document Detail

Translational inhibitors cycloheximide, emetine, and puromycin inhibit cellular autophagy in mouse liver parenchymal and pancreatic acinar cells in vivo.
MedLine Citation:
PMID:  1789148     Owner:  NLM     Status:  MEDLINE    
The protein synthesis inhibitor cycloheximide is widely used (in vitro or in vivo) to inhibit the autophagic degradation of endogenous cellular proteins. Circumstantial evidence has been obtained largely from in vitro experiments for a similar effect of other translational inhibitors. In the present study, the in vivo effects of cycloheximide, emetine and puromycin on autophagy in murine exocrine pancreatic and liver cells were tested using electron microscopic morphometry. The experiments were based on the assumption that the autophagic compartment will regress if the formation of the vacuoles is blocked while degradation in the pre-existing vacuoles goes on. To make the measurements easier, autophagic compartment of the cells was greatly enlarged in both cell types by administering vinblastine (10 mg/kg b. wt.) for 2 h when the inhibitors were set on for an additional 30 min. During this half-an-hour, cycloheximide (0.2 mg/g b. wt.), emetine (0.12 mg/g b. wt.) and puromycin (0.2 mg/g b. wt.), respectively caused 58.5, 35.6, and 69.5% regression of the pancreatic and 46.7, 64.2, and 54.2% of the hepatocytic autophagic vacuole compartment. Thus, similarly to cycloheximide, both emetine and puromycin have proved to be inhibitors of autophagy in vivo. The results argue for a possible relationship between the synthesis and degradation of endogenous cellular proteins.
O Oliva; L László; Z Pálfia; G Réz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta morphologica Hungarica     Volume:  39     ISSN:  0236-5391     ISO Abbreviation:  Acta Morphol Hung     Publication Date:  1991  
Date Detail:
Created Date:  1992-03-26     Completed Date:  1992-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8307964     Medline TA:  Acta Morphol Hung     Country:  HUNGARY    
Other Details:
Languages:  eng     Pagination:  79-85     Citation Subset:  IM    
Dept. of General Zoology, Eötvös Univ. Budapest, Hungary.
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MeSH Terms
Autophagy / drug effects*
Cycloheximide / pharmacology
Emetine / pharmacology
Liver / cytology,  drug effects*,  ultrastructure
Microscopy, Electron
Pancreas / cytology,  drug effects*,  ultrastructure
Protein Biosynthesis / drug effects
Protein Synthesis Inhibitors / pharmacology*
Puromycin / pharmacology
Vinblastine / pharmacology
Reg. No./Substance:
0/Protein Synthesis Inhibitors; 483-18-1/Emetine; 53-79-2/Puromycin; 66-81-9/Cycloheximide; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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