| Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog. | |
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MedLine Citation:
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PMID: 17611220 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Translational control is crucial for proper timing of developmental events that take place in the absence of transcription, as in meiotic activation in oocytes, early embryogenesis in many organisms, and spermatogenesis. Here we show that a novel form of the translation initiation complex component eIF4G in Drosophila, eIF4G2, is required specifically for male germ cells to undergo meiotic division and proper spermatid differentiation. Flies mutant for eIF4G2 are viable and female fertile but male sterile. Spermatocytes form, but the germ cells in mutant males skip the major events of the meiotic divisions and form aberrant spermatids with large nuclei. Consistent with the failure to undergo the meiotic divisions, function of eIF4G2 is required post-transcriptionally for normal accumulation of the core cell cycle regulatory proteins Twine and CycB in mature spermatocytes. Loss of eIF4G2 function also causes widespread defects in spermatid differentiation. Although differentiation markers Dj and Fzo are expressed in late-stage eIF4G2 mutant germ cells, several key steps of spermatid differentiation fail, including formation of a compact mitochondrial derivative and full elongation. Our results suggest that an alternate form of the translation initiation machinery may be required for regulation and execution of key steps in male germ cell differentiation. |
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Authors:
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Catherine C Baker; Margaret T Fuller |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-07-04 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 134 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-11 Completed Date: 2007-09-28 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 2863-9 Citation Subset: IM |
Affiliation:
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Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5329, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Cell Cycle / genetics Cell Differentiation* / genetics Cells, Cultured Drosophila / genetics Drosophila Proteins / genetics, physiology* Eukaryotic Initiation Factor-4G / genetics, physiology* Fertility / genetics Gene Expression Regulation, Developmental* Male Meiosis / genetics* Protein Biosynthesis* Sequence Homology, Amino Acid Spermatids / cytology* Spermatozoa / cytology* Testis / growth & development |
| Grant Support | |
ID/Acronym/Agency:
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1 F32 GM071260/GM/NIGMS NIH HHS; T32 HD007249/HD/NICHD NIH HHS; U54-HD31398/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Eukaryotic Initiation Factor-4G; 0/off-schedule protein, Drosophila |
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