Document Detail


Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog.
MedLine Citation:
PMID:  17611220     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Translational control is crucial for proper timing of developmental events that take place in the absence of transcription, as in meiotic activation in oocytes, early embryogenesis in many organisms, and spermatogenesis. Here we show that a novel form of the translation initiation complex component eIF4G in Drosophila, eIF4G2, is required specifically for male germ cells to undergo meiotic division and proper spermatid differentiation. Flies mutant for eIF4G2 are viable and female fertile but male sterile. Spermatocytes form, but the germ cells in mutant males skip the major events of the meiotic divisions and form aberrant spermatids with large nuclei. Consistent with the failure to undergo the meiotic divisions, function of eIF4G2 is required post-transcriptionally for normal accumulation of the core cell cycle regulatory proteins Twine and CycB in mature spermatocytes. Loss of eIF4G2 function also causes widespread defects in spermatid differentiation. Although differentiation markers Dj and Fzo are expressed in late-stage eIF4G2 mutant germ cells, several key steps of spermatid differentiation fail, including formation of a compact mitochondrial derivative and full elongation. Our results suggest that an alternate form of the translation initiation machinery may be required for regulation and execution of key steps in male germ cell differentiation.
Authors:
Catherine C Baker; Margaret T Fuller
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-07-04
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  134     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-09-28     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2863-9     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Cell Cycle / genetics
Cell Differentiation* / genetics
Cells, Cultured
Drosophila / genetics
Drosophila Proteins / genetics,  physiology*
Eukaryotic Initiation Factor-4G / genetics,  physiology*
Fertility / genetics
Gene Expression Regulation, Developmental*
Male
Meiosis / genetics*
Protein Biosynthesis*
Sequence Homology, Amino Acid
Spermatids / cytology*
Spermatozoa / cytology*
Testis / growth & development
Grant Support
ID/Acronym/Agency:
1 F32 GM071260/GM/NIGMS NIH HHS; T32 HD007249/HD/NICHD NIH HHS; U54-HD31398/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Eukaryotic Initiation Factor-4G; 0/off-schedule protein, Drosophila

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