Document Detail


Translational control of JunB, an AP-1 transcription factor, in activated human endothelial cells.
MedLine Citation:
PMID:  23297064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stimulated endothelial cells (EC) assume an activated phenotype with pro-inflammatory and prothrombotic features, requiring new gene and protein expression. New protein synthesis in activated EC is largely regulated by transcriptional events controlled by a variety of transcription factors. However, post-transcriptional control of gene expression also influences phenotype and allows the cell to alter protein expression in a faster and more direct way than is typically possible with transcriptional mechanisms. We sought to demonstrate that post-transcriptional control of gene expression occurs during EC activation. Using thrombin-activated EC and a high-throughput, microarray-based approach, we identified a number of gene products that may be regulated through post-transcriptional mechanisms, including the AP-1 transcription factor JunB. Using polysome profiling, cytoplasts and other standard cell biologic techniques, JunB is shown to be regulated at a post-transcriptional level during EC activation. In activated EC, the AP-1 transcription factor JunB, is regulated on a post-transcriptional level. Signal-dependent control of translation may regulate transcription factor expression and therefore, subsequent transcriptional events in stimulated EC.
Authors:
Douglas I Schmid; Hansjörg Schwertz; Huimiao Jiang; Robert A Campbell; Andrew S Weyrich; Thomas M McIntyre; Guy A Zimmerman; Larry W Kraiss
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  114     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-05-13     Completed Date:  2013-10-29     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1519-28     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Gene Expression Regulation / genetics,  physiology
Human Umbilical Vein Endothelial Cells / metabolism*
Humans
Oligonucleotide Array Sequence Analysis
Protein Processing, Post-Translational / genetics,  physiology
Transcription Factor AP-1 / genetics,  metabolism*
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
HL 075507/HL/NHLBI NIH HHS; HL 66277/HL/NHLBI NIH HHS; R01 HL066277/HL/NHLBI NIH HHS; R37HL 44525/HL/NHLBI NIH HHS; U54 HL112311/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/JunB protein, human; 0/Transcription Factor AP-1; 0/Transcription Factors
Comments/Corrections

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