Document Detail


Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform.
MedLine Citation:
PMID:  15601834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mRNA cap-binding protein eukaryotic translation initiation factor 4E (eIF4E) participates in protein synthesis initiation, translational repression of specific mRNAs, and nucleocytoplasmic shuttling. Multiple isoforms of eIF4E are expressed in a variety of organisms, but their specific roles are poorly understood. We investigated one Caenorhabditis elegans isoform, IFE-4, which has homologues in plants and mammals. IFE-4::green fluorescent protein (GFP) was expressed in pharyngeal and tail neurons, body wall muscle, spermatheca, and vulva. Knockout of ife-4 by RNA interference (RNAi) or a null mutation produced a pleiotropic phenotype that included egg-laying defects. Sedimentation analysis demonstrated that IFE-4, but not IFE-1, was present in 48S initiation complexes, indicating that it participates in protein synthesis initiation. mRNAs affected by ife-4 knockout were determined by DNA microarray analysis of polysomal distribution. Polysome shifts, in the absence of total mRNA changes, were observed for only 33 of the 18,967 C. elegans mRNAs tested, of which a disproportionate number were related to egg laying and were expressed in neurons and/or muscle. Translational regulation was confirmed by reduced levels of DAF-12, EGL-15, and KIN-29. The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E.
Authors:
Tzvetanka D Dinkova; Brett D Keiper; Nadejda L Korneeva; Eric J Aamodt; Robert E Rhoads
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  25     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-16     Completed Date:  2005-01-18     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  100-13     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Animals, Genetically Modified
Caenorhabditis elegans
Caenorhabditis elegans Proteins / biosynthesis,  physiology
Cell Nucleus / metabolism
Centrifugation, Density Gradient
Chromosome Mapping
Crosses, Genetic
Cytoplasm / metabolism
Eukaryotic Initiation Factor-4E / chemistry*,  metabolism
Gene Deletion
Gene Expression Regulation
Green Fluorescent Proteins / metabolism
Homozygote
Mice
Mice, Knockout
Models, Genetic
Muscles / metabolism
Mutation
Neurons / metabolism
Oligonucleotide Array Sequence Analysis
Peptide Initiation Factors / physiology
Phenotype
Polyribosomes / metabolism
Protein Binding
Protein Biosynthesis*
Protein Isoforms
Protein-Serine-Threonine Kinases / biosynthesis
RNA / metabolism
RNA Interference
RNA, Messenger / metabolism*
Receptors, Cytoplasmic and Nuclear / biosynthesis
Receptors, Fibroblast Growth Factor / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Sucrose / pharmacology
Time Factors
Grant Support
ID/Acronym/Agency:
GM 20818/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/DAF-12 protein, C elegans; 0/EGL-15 protein, C elegans; 0/Eukaryotic Initiation Factor-4E; 0/Peptide Initiation Factors; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Fibroblast Growth Factor; 147336-22-9/Green Fluorescent Proteins; 57-50-1/Sucrose; 63231-63-0/RNA; EC 2.7.1.-/kin-29 protein, C elegans; EC 2.7.11.1/Protein-Serine-Threonine Kinases
Comments/Corrections

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