| Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts. | |
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MedLine Citation:
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PMID: 4506788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Single-stranded reovirus RNA, synthesized in vitro by reovirus cores, functioned as messenger RNA in cell-free extracts prepared from several mammalian cells: Krebs II mouse ascites cells, mouse L cells, Chinese hamster ovary cells, HeLa cells, and rabbit reticulocytes. As shown by acrylamide gel electrophoresis, all eight polypeptides known to be specified by reovirus were synthesized in the reticulocyte system. In the other extracts, from 5 to 7 complete virus proteins were made. |
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Authors:
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M J McDowell; W K Joklik; L Villa-Komaroff; H F Lodish |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 69 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1972 Sep |
Date Detail:
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Created Date: 1972-11-10 Completed Date: 1972-11-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2649-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Carbon Isotopes Carcinoma, Krebs 2 / metabolism* Cell Line Cricetinae Electrophoresis Female Hela Cells / drug effects, metabolism* L Cells (Cell Line) / drug effects, metabolism* Leucine / metabolism Methionine / metabolism Mice Ovary / drug effects, metabolism* RNA, Messenger / pharmacology* RNA, Viral / pharmacology* Rabbits Reoviridae* Reticulocytes / drug effects, metabolism* Sodium Dodecyl Sulfate Sulfur Isotopes Tritium Viral Proteins / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/RNA, Messenger; 0/RNA, Viral; 0/Sulfur Isotopes; 0/Viral Proteins; 10028-17-8/Tritium; 151-21-3/Sodium Dodecyl Sulfate; 61-90-5/Leucine; 63-68-3/Methionine |
| Comments/Corrections | |
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