| Translation initiation factor eIF-4G is immunogenic, overexpressed, and amplified in patients with squamous cell lung carcinoma. | |
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MedLine Citation:
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PMID: 11550153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Recently, the authors reported identification and cloning of several novel immunogenic antigens in squamous cell lung carcinoma. Of 14 corresponding genes, 9 mapped in an amplified chromosomal region with the gene for eIF-4G that was amplified most frequently. METHODS: Recombinant eIF-4G was expressed in E. coli and screened with sera from patients with squamous cell carcinoma of the lung and of the head and neck. Protein extracts from squamous cell carcinoma tissues were analyzed for eIF-4G expression by Western blot analysis. Mutation analysis was performed using an automatic DNA sequencer. RESULTS: The authors screened a spectrum of 33 heterologous sera from lung carcinoma patients and detected antibodies against eIF-4G in 5 of these sera (15%). They found no immune response to eIF-4G in 17 sera from squamous cell carcinoma tissues derived from the head and neck. In addition, no antibodies were found to eIF-4G in a group of 17 control sera from individuals without known tumor formation. Sequence analysis of the eIF-4G gave no indication of mutations. To analyze the expression of eIF-4G, a monoclonal antibody was used. Western blot analysis clearly showed overexpression in the tumor tissue compared with the corresponding normal lung tissues. CONCLUSIONS: The translation initiation factor eIF-4G is the first protein in which the gene shows amplification, has increased expression in a human tumor, and induces an immune response in patients. eIF-4G lends itself as a marker for the diagnosis of and possibly as a future therapeutic target in patients with squamous cell lung carcinoma. |
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Authors:
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C Bauer; I Diesinger; N Brass; H Steinhart; H Iro; E U Meese |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer Volume: 92 ISSN: 0008-543X ISO Abbreviation: Cancer Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-09-10 Completed Date: 2001-09-20 Revised Date: 2006-04-21 |
Medline Journal Info:
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Nlm Unique ID: 0374236 Medline TA: Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 822-9 Citation Subset: AIM; IM |
Copyright Information:
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Copyright 2001 American Cancer Society. |
Affiliation:
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Department of Human Genetics, Medical School, University of Saarland, 66421 Homburg/Saar, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibody Formation Antigens, Neoplasm Blotting, Western Carcinoma, Squamous Cell / diagnosis, genetics*, metabolism DNA / isolation & purification DNA, Complementary Eukaryotic Initiation Factor-4G Gene Amplification Genetic Markers Genetic Vectors Humans Lung Neoplasms / diagnosis, genetics*, metabolism Peptide Initiation Factors* / genetics, immunology Protein Biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/DNA, Complementary; 0/Eukaryotic Initiation Factor-4G; 0/Genetic Markers; 0/Peptide Initiation Factors; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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