Document Detail


Translation and functional expression of cell-cell channel mRNA in Xenopus oocytes.
MedLine Citation:
PMID:  2416937     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
mRNA from estrogen-stimulated rat myometrium, a tissue known to upregulate cell-cell channels in response to this hormone, was microinjected into Xenopus laevis oocytes. The oocytes had been freed from covering layers of follicle cells and vitelline to allow direct cell membrane interactions when paired. About 4 hours after the mRNA injection, paired oocytes become electrically coupled. This coupling was due to the presence of typical cell-cell channels characterized by size-limited intercellular tracer flux, the presence of gap junctions at the oocyte-oocyte interface, and the reversible uncoupling that occurred in the presence of carbon dioxide. The induction of new cell-cell channels in the oocyte membrane was observed against a zero background or a low level of endogenous coupling, depending on the maturation stage of the oocytes. The time course of development of cell-cell coupling after the microinjection of mRNA was determined. The mRNA capable of inducing cell-cell coupling was confined to an intermediate size class when fractionated on a sucrose gradient.
Authors:
R Werner; T Miller; R Azarnia; G Dahl
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  87     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  1985  
Date Detail:
Created Date:  1986-02-19     Completed Date:  1986-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-68     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication*
Female
Freeze Fracturing
Ion Channels / physiology*,  ultrastructure
Microinjections
Microscopy, Electron
Myometrium / physiology
Oocytes / physiology*,  ultrastructure
Protein Biosynthesis*
RNA, Messenger / genetics*
Rats
Rats, Inbred Strains
Sulfates / metabolism
Xenopus
Chemical
Reg. No./Substance:
0/Ion Channels; 0/RNA, Messenger; 0/Sulfates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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