| Translation and functional expression of cell-cell channel mRNA in Xenopus oocytes. | |
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MedLine Citation:
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PMID: 2416937 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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mRNA from estrogen-stimulated rat myometrium, a tissue known to upregulate cell-cell channels in response to this hormone, was microinjected into Xenopus laevis oocytes. The oocytes had been freed from covering layers of follicle cells and vitelline to allow direct cell membrane interactions when paired. About 4 hours after the mRNA injection, paired oocytes become electrically coupled. This coupling was due to the presence of typical cell-cell channels characterized by size-limited intercellular tracer flux, the presence of gap junctions at the oocyte-oocyte interface, and the reversible uncoupling that occurred in the presence of carbon dioxide. The induction of new cell-cell channels in the oocyte membrane was observed against a zero background or a low level of endogenous coupling, depending on the maturation stage of the oocytes. The time course of development of cell-cell coupling after the microinjection of mRNA was determined. The mRNA capable of inducing cell-cell coupling was confined to an intermediate size class when fractionated on a sucrose gradient. |
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Authors:
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R Werner; T Miller; R Azarnia; G Dahl |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of membrane biology Volume: 87 ISSN: 0022-2631 ISO Abbreviation: J. Membr. Biol. Publication Date: 1985 |
Date Detail:
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Created Date: 1986-02-19 Completed Date: 1986-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 253-68 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Communication* Female Freeze Fracturing Ion Channels / physiology*, ultrastructure Microinjections Microscopy, Electron Myometrium / physiology Oocytes / physiology*, ultrastructure Protein Biosynthesis* RNA, Messenger / genetics* Rats Rats, Inbred Strains Sulfates / metabolism Xenopus |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 0/RNA, Messenger; 0/Sulfates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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