| Translation factor LepA contributes to tellurite resistance in Escherichia coli but plays no apparent role in the fidelity of protein synthesis. | |
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MedLine Citation:
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PMID: 19925844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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LepA is a translational GTPase highly conserved in bacterial lineages. While it has been shown that LepA can catalyze reverse ribosomal translocation in vitro, the role of LepA in the cell remains unclear. Here, we show that deletion of the lepA gene (DeltalepA) in Escherichia coli causes hypersensitivity to potassium tellurite and penicillin G, but has no appreciable effect on growth under many other conditions. DeltalepA does not increase miscoding or frameshifting errors under normal or stress conditions, indicating that LepA does not contribute to the fidelity of translation. Overexpression of LepA interferes with tmRNA-mediated peptide tagging and A-site mRNA cleavage, suggesting that LepA is a bona fide translation factor that can act on stalled ribosomes with a vacant A site in vivo. Together these results lead us to hypothesize that LepA is involved in co-translational folding of proteins that are otherwise vulnerable to tellurite oxidation. |
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Authors:
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Shinichiro Shoji; Brian D Janssen; Christopher S Hayes; Kurt Fredrick |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-11-17 |
Journal Detail:
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Title: Biochimie Volume: 92 ISSN: 1638-6183 ISO Abbreviation: Biochimie Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-26 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 157-63 Citation Subset: IM |
Copyright Information:
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2009 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Department of Microbiology, The Ohio State University, Columbus, 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Drug Resistance, Bacterial* Escherichia coli / cytology, drug effects*, genetics, metabolism* Escherichia coli Proteins / biosynthesis*, genetics, metabolism GTP Phosphohydrolases / deficiency, genetics Gene Deletion Oxidants / pharmacology Phosphoproteins / deficiency, genetics Protein Biosynthesis / physiology* RNA, Messenger / genetics Tellurium / pharmacology* Transcriptional Elongation Factors / deficiency, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM072528/GM/NIGMS NIH HHS; GM078634/GM/NIGMS NIH HHS; R01 GM072528-05/GM/NIGMS NIH HHS; R01 GM078634-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins; 0/LepA protein, E coli; 0/Oxidants; 0/Phosphoproteins; 0/RNA, Messenger; 0/Transcriptional Elongation Factors; 13494-80-9/Tellurium; 7790-58-1/potassium tellurate(IV); EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/typA protein, E coli |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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