Document Detail

Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in cardiomyocytes through a Calcineurin-dependent pathway.
MedLine Citation:
PMID:  22850285     Owner:  NLM     Status:  Publisher    
The Myocyte Enhancer Factor-2 (MEF2) family of transcription factors regulates gene expression during cardiomyocyte differentiation and adaptation of the myocardium to stress. MEF2 activity is enhanced by increasing its transcription and by MAPK-dependent phosphorylation, and is reduced by binding to class-II Histone Deacetylases and by miR-1-mediated degradation of its transcript. Here we show that MEF2 protein abundance is regulated at the translational level, determining myocyte size, during hypertrophy. In order to reduce MEF2 protein expression, its silencing through RNA interference required serum deprivation and, even in this condition, MEF2 protein abundance recovered to basal levels in presence of phenylephrine. Hypertrophic agonist stimulation of neonatal ventricular cardiomyocytes increased Mef2 expression by enhancing its translation, without changing its transcription or blocking degradation of the protein. MEF2 abundance was increased by Calcineurin overexpression in vivo and was reduced by Calcineurin inhibition in vitro, without affecting Mef2 mRNA levels. Calcineurin activity influenced expression of Polypyrimidine Tract Protein (PTB), contributing to MEF2 translation. Thus, our results show a previously unrecognized but relevant level of MEF2 activity regulation through the control of its translation that involves Calcineurin and PTB.
Junmei Ye; Maria Cardona; Marta Llovera; Joan X Comella; Daniel Sanchis
Related Documents :
19912915 - Testosterone induces an all-or-none, exponential increase in arginine vasopressin mrna ...
22607375 - Increasing levels of wild-type creb up-regulates several activity-regulated inhibitor o...
10366415 - Pg2 gene expression and its regulation in human adrenocortical and medullary tumors.
9348205 - Regulation of corticotropin-releasing factor (crf) messenger ribonucleic acid and crf p...
24391115 - The induction of lipocalin-2 protein expression in vivo and in vitro.
9858575 - Fiber-type-specific transcription of the troponin i slow gene is regulated by multiple ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-28
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  -     ISSN:  1095-8584     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Institut de Recerca Biomèdica de Lleida (IRBLLEIDA) - Universitat de Lleida, Edifici Biomedicina-I. Av. Rovira Roure, 80. 25198 Lleida, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stem cell factor is responsible for the rapid response in mature mast cell density in the acutely st...
Next Document:  Myosin binding protein-C phosphorylation is the principal mediator of protein kinase A effects on th...