Document Detail


Transitory acidification of semen as a potential method for the inactivation of some pathogenic microorganisms. Effect on fertilization and development of ova in superovulated heifers.
MedLine Citation:
PMID:  16726975     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The fertilizing capacity of transitorily acidified semen was investigated with respect to using acidification as a method for destroying or inactivating acid labile pathogenic microorganisms in semen prior to freezing. Ejaculates diluted 1:1 with phosphate buffered saline (PBS) were acidified to pH 5.0 for 2 or 5 minutes before being returned to their original pH. They were then frozen by commercial methods and used to artificially inseminate superovulated heifers. A total 739 ova and embryos was collected from 119 inseminated donors. The fertilization rate was 88%, and 78% of the embryos were of transferable quality. Semen acidification had no apparent effect on the post-thaw percentage of intact acrosomes.
Authors:
A Bielanski; P Eastman; W C Hare
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  36     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1991 Jul 
Date Detail:
Created Date:  2006-05-26     Completed Date:  2009-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  33-40     Citation Subset:  -    
Affiliation:
Agriculture Canada, Animal Diseases Research Institute, NEPEAN, P.O.Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.
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