| Transitions in ventricular activation revealed by two-dimensional optical mapping. | |
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MedLine Citation:
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PMID: 15372433 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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While cardiac function in the mature heart is dependent on a properly functioning His-Purkinje system, the early embryonic tubular heart efficiently pumps blood without a distinct specialized conduction system. Although His-Purkinje system precursors have been identified using immunohistological techniques in the looped heart, little is known whether these precursors function electrically. To address this question, we used high-resolution optical mapping and fluorescent dyes with two CCD cameras to describe the motion-corrected activation patterns of 76 embryonic chick hearts from tubular stages (stage 10) to mature septated hearts (stage 35). Ventricular activation in the tubular looped heart (stages 10-17) using both calcium-sensitive fluo-4 and voltage-sensitive di-4-ANEPPS shows sequentially uniform propagation. In late looped hearts (stages 18-22), domains of the dorsal and lateral ventricle are preferentially activated before spreading to the remaining myocardium and show alternating regions of fast and slow propagation. During stages 22-26, action potentials arise from the dorsal ventricle. By stages 27-29, action potential breakthrough is also observed at the right ventricle apex. By stage 31, activation of the heart proceeds from foci at the apex and dorsal surface of the heart. The breakthrough foci correspond to regions where putative conduction system precursors have been identified immunohistologically. To date, our study represents the most detailed electrophysiological characterization of the embryonic heart between the looped and preseptated stages and suggests that ventricular activation undergoes a gradual transformation from sequential to a mature pattern with right and left epicardial breakthroughs. Our investigation suggests that cardiac conduction system precursors may be electrophysiologically distinct and mature gradually throughout cardiac morphogenesis in the chick. |
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Authors:
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Emil Thomas Chuck; Kathleen Meyers; David France; Tony L Creazzo; Gregory E Morley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology Volume: 280 ISSN: 1552-4884 ISO Abbreviation: - Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-10-06 Completed Date: 2005-02-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101234285 Medline TA: Anat Rec A Discov Mol Cell Evol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 990-1000 Citation Subset: IM |
Copyright Information:
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copyright 2004 Wiley-Liss, Inc. |
Affiliation:
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Neonatal-Perinatal Research Institute, Division of Neonatology, Department of Pediatrics and Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials* Animals Calcium / metabolism Chick Embryo* / anatomy & histology, physiology Electrophysiology Fluorescent Dyes / diagnostic use Heart Conduction System / anatomy & histology, embryology*, metabolism Heart Ventricles / anatomy & histology, embryology*, metabolism* Myocardium / cytology*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL-36059/HL/NHLBI NIH HHS; HL-39707/HL/NHLBI NIH HHS; HL-58861/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 7440-70-2/Calcium |
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