| Transitions from alkaline spots to regular bands during pH pattern formation at the plasmalemma of Chara cells. | |
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MedLine Citation:
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PMID: 12734703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A scanning pH-microprobe was used to study pH patterns near the surface of Chara corallina cells at various light intensities and during light-induced transitions from homogeneous pH distribution to alternating pH bands. In the irradiance (PAR) range 4-400 micromol quanta m(-2) s(-1), the sustained pH profiles consisted of alternating acid and alkaline bands with a characteristic length of 7-10 mm and pH shifts as large as 2-3 units. At lower irradiance, the number of alkaline bands decreased while the amplitude of remaining peaks stayed high. On cyclic changes in light intensity, a hysteresis of pH banding was observed: the pH bands tolerated low irradiance in weakening light, but higher irradiance was required for their emergence after dark adaptation of the cell. The pH profiles measured for different paths of electrode scanning suggest that the pH pattern at low light level represents patches coexisting with bands. The exposure of the cell to high-intensity light led to formation of radially symmetrical bands. Transformations of the pH pattern induced by lowering the light intensity were similar to those induced by transcellular electric current (1.5-3 microA). The data suggest that band formation at the plasmalemma of Chara cells proceeds through the initial appearance of multiple patches with a localized H(+)-transporting activity and subsequent spot rearrangements (fusion, deletions, widening), leading to establishment of alternating bands. |
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Authors:
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A A Bulychev; S V Zykov; A B Rubin; S C Müller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-03-06 |
Journal Detail:
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Title: European biophysics journal : EBJ Volume: 32 ISSN: 0175-7571 ISO Abbreviation: Eur. Biophys. J. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-07 Completed Date: 2004-03-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8409413 Medline TA: Eur Biophys J Country: Germany |
Other Details:
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Languages: eng Pagination: 144-53 Citation Subset: IM |
Affiliation:
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Biophysics Department, Faculty of Biology, Moscow State University, 119899 Moscow, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Cell Membrane / chemistry*, radiation effects* Cells, Cultured Chara / chemistry*, radiation effects* Dose-Response Relationship, Radiation Electromagnetic Fields Hydrogen-Ion Concentration Light* Molecular Probes Nonlinear Dynamics |
| Chemical | |
Reg. No./Substance:
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0/Molecular Probes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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