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Transition to annual life history coincides with reduction in cell cycle speed during early cleavage in three independent clades of annual killifish.
MedLine Citation:
PMID:  25276337     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Annual killifishes inhabit temporary ponds and their embryos survive the dry season encased in the mud by entering diapause, a process that arrests embryonic development during hostile conditions. Annual killifishes are present within three clades distributed in Africa (one East and one West of the Dahomey gap) and South America. Within each of these phylogenetic clades, a non-annual clade is sister taxon to a annual clade and therefore represent an example of convergent evolution. Early cleavage of teleost embryos is characterized by a very fast cell cycle (15-30 minutes) and lack of G1 and G2 phases. Here, we decided to investigate rates of early cleavage in annual killifishes. In addition, we specifically tested whether also annual killifish embryos lack G1 and G2 phases.
RESULTS: We used time lapse brightfield microscopy to investigate cell division kinetics during the first developmental stages of annual- and non-annual species belonging to the three different phylogenetic clades. Annual killifishes of all three clades showed cleavage times significantly longer when compared to their non-annual sister taxa (average 35 min vs. average 75 min). Using FUCCI fluorescent imaging of the cell cycle after microinjection in the annual species Nothobranchius furzeri, we demonstrate that the first 5 division are synchronous and do not show a G1 phase. Cell cycle synchronization is lost after the 5th cleavage division.
CONCLUSIONS: Our results show, for the first time, that cell cycle rate during cleavage, a trait thought to be rather evolutionary conserved can undergo convergent evolutionary change in response to variations in life-history.
Authors:
Luca Dolfi; Roberto Ripa; Alessandro Cellerino
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Publication Detail:
Type:  Journal Article     Date:  2014-09-22
Journal Detail:
Title:  EvoDevo     Volume:  5     ISSN:  2041-9139     ISO Abbreviation:  Evodevo     Publication Date:  2014  
Date Detail:
Created Date:  2014-10-03     Completed Date:  2014-10-03     Revised Date:  2014-10-04    
Medline Journal Info:
Nlm Unique ID:  101525836     Medline TA:  Evodevo     Country:  England    
Other Details:
Languages:  eng     Pagination:  32     Citation Subset:  -    
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